Topoisomerase II (TOP2)

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DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme

Topoisomerase II (TOP2)

Type II topoisomerase is an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA792Hu01 Recombinant Topoisomerase II (TOP2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA792Hu01 Polyclonal Antibody to Topoisomerase II (TOP2) WB; IHC; ICC; IP.
MAA792Hu22 Monoclonal Antibody to Topoisomerase II (TOP2) WB; IHC; ICC; IP.
Assay Kits SEA792Hu ELISA Kit for Topoisomerase II (TOP2) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA792Mu01 Recombinant Topoisomerase II (TOP2) Positive Control; Immunogen; SDS-PAGE; WB.
RPA792Mu02 Recombinant Topoisomerase II (TOP2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA792Mu01 Polyclonal Antibody to Topoisomerase II (TOP2) WB; IHC; ICC; IP.
PAA792Mu02 Polyclonal Antibody to Topoisomerase II (TOP2) WB; IHC; ICC; IP.
Assay Kits SEA792Mu ELISA Kit for Topoisomerase II (TOP2) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA792Ra01 Recombinant Topoisomerase II (TOP2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA792Ra01 Polyclonal Antibody to Topoisomerase II (TOP2) WB; IHC; ICC; IP.
MAA792Ra21 Monoclonal Antibody to Topoisomerase II (TOP2) WB; IHC; ICC; IP.
Assay Kits SEA792Ra ELISA Kit for Topoisomerase II (TOP2) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22."Proc. Natl. Acad. Sci. U.S.A. 85:7177-7181(1988) [PubMed] [Europe PMC] [Abstract]
  2. "Use of yeast in the study of anticancer drugs targeting DNA topoisomerases: expression of a functional recombinant human DNA topoisomerase II alpha in yeast."Cancer Res. 53:3591-3596(1993) [PubMed] [Europe PMC] [Abstract]
  3. "Structural organization of the human TOP2A and TOP2B genes."Gene 221:255-266(1998) [PubMed] [Europe PMC] [Abstract]
  4. "Molecular cloning and characterization of the human topoisomerase IIalpha and IIbeta genes: evidence for isoform evolution through gene duplication."Biochim. Biophys. Acta 1444:395-406(1999) [PubMed] [Europe PMC] [Abstract]
  5. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]