Poly ADP Ribose Polymerase (PARP)

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PARP1; ADPRT; ADPRT1; PPOL; pADPRT-1; ADP-ribosyltransferase diphtheria toxin-like 1; NAD(+) ADP-ribosyltransferase 1

Poly ADP Ribose Polymerase (PARP)
is a protein involved in a number of cellular processes involving mainly DNA repair and programmed cell death.RP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress . This protein can be cleaved by many ICE-like caspases in vitroand is one of the main cleavage targets of caspase-3 in vivo . In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) .
PARP1 is a chromatin-associated enzyme, poly-ADP ribose polymerase-1, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA279Hu01 Recombinant Poly ADP Ribose Polymerase (PARP) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA279Hu01 Polyclonal Antibody to Poly ADP Ribose Polymerase (PARP) WB
MAA279Hu21 Monoclonal Antibody to Poly ADP Ribose Polymerase (PARP) WB
MAA279Hu22 Monoclonal Antibody to Poly ADP Ribose Polymerase (PARP) WB
MAA279Hu23 Monoclonal Antibody to Poly ADP Ribose Polymerase (PARP) WB
MAA279Hu24 Monoclonal Antibody to Poly ADP Ribose Polymerase (PARP) WB
MAA279Hu25 Monoclonal Antibody to Poly ADP Ribose Polymerase (PARP) WB
Assay Kits SEA279Hu ELISA Kit for Poly ADP Ribose Polymerase (PARP) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA279Hu CLIA Kit for Poly ADP Ribose Polymerase (PARP) Chemiluminescent immunoassay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA279Mu01 Recombinant Poly ADP Ribose Polymerase (PARP) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA279Mu01 Polyclonal Antibody to Poly ADP Ribose Polymerase (PARP) WB; IHC; FCM
Assay Kits SEA279Mu ELISA Kit for Poly ADP Ribose Polymerase (PARP) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Poly ADP Ribose Polymerase (PARP) Recombinant Protein Customized Service Offer
Antibodies PAA279Ra01 Polyclonal Antibody to Poly ADP Ribose Polymerase (PARP) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Poly ADP Ribose Polymerase (PARP) CLIA Kit Customized Service Offer
n/a ELISA Kit for Poly ADP Ribose Polymerase (PARP) ELISA Kit Customized Service Offer
  1. "Nucleotide sequence of a full-length cDNA for human fibroblast poly(ADP-ribose) polymerase."Biochem. Biophys. Res. Commun. 148:617-622(1987) [PubMed] [Europe PMC] [Abstract]
  2. "Primary structure of human poly(ADP-ribose) synthetase as deduced from cDNA sequence."J. Biol. Chem. 262:15990-15997(1987) [PubMed] [Europe PMC] [Abstract]
  3. "cDNA sequence, protein structure, and chromosomal location of the human gene for poly(ADP-ribose) polymerase."Proc. Natl. Acad. Sci. U.S.A. 84:8370-8374(1987) [PubMed] [Europe PMC] [Abstract]
  4. "Human nuclear NAD+ ADP-ribosyltransferase(polymerizing): organization of the gene."DNA 8:575-580(1989) [PubMed] [Europe PMC] [Abstract]
  5. "The DNA sequence and biological annotation of human chromosome 1." Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Human poly(ADP-ribose) polymerase gene. Cloning of the promoter region."Eur. J. Biochem. 194:521-526(1990) [PubMed] [Europe PMC] [Abstract]
  8. "Characterization of a putative promoter region of the human poly(ADP-ribose) polymerase gene: structural similarity to that of the DNA polymerase beta gene."Biochem. Biophys. Res. Commun. 167:701-710(1990) [PubMed] [Europe PMC] [Abstract]
  9. , Submitted (DEC-2008) to UniProtKB
  10. "Txk, a member of the non-receptor tyrosine kinase of the Tec family, forms a complex with poly(ADP-ribose) polymerase 1 and elongation factor 1alpha and regulates interferon-gamma gene transcription in Th1 cells."Clin. Exp. Immunol. 147:164-175(2007) [PubMed] [Europe PMC] [Abstract]
  11. "Isolation of a cDNA clone for human NAD+: protein ADP-ribosyltransferase."Eur. J. Cell Biol. 44:302-307(1987) [PubMed] [Europe PMC] [Abstract]
  12. "Molecular cloning of cDNA for human poly(ADP-ribose) polymerase and expression of its gene during HL-60 cell differentiation."Biochem. Biophys. Res. Commun. 146:403-409(1987) [PubMed] [Europe PMC] [Abstract]
  13. ErratumBiochem. Biophys. Res. Commun. 148:1549-1550(1987)
  14. "The second zinc-finger domain of poly(ADP-ribose) polymerase determines specificity for single-stranded breaks in DNA."Proc. Natl. Acad. Sci. U.S.A. 87:2990-2994(1990) [PubMed] [Europe PMC] [Abstract]
  15. "The zinc fingers of human poly(ADP-ribose) polymerase are differentially required for the recognition of DNA breaks and nicks and the consequent enzyme activation. Other structures recognize intact DNA."J. Biol. Chem. 265:21907-21913(1990) [PubMed] [Europe PMC] [Abstract]
  16. "Expression and site-directed mutagenesis of the catalytic domain of human poly(ADP-ribose)polymerase in Escherichia coli. Lysine 893 is critical for activity."J. Biol. Chem. 265:19249-19256(1990) [PubMed] [Europe PMC] [Abstract]
  17. "The human poly(ADP-ribose) polymerase nuclear localization signal is a bipartite element functionally separate from DNA binding and catalytic activity."EMBO J. 11:3263-3269(1992) [PubMed] [Europe PMC] [Abstract]
  18. "Random mutagenesis of the poly(ADP-ribose) polymerase catalytic domain reveals amino acids involved in polymer branching."Biochemistry 36:12147-12154(1997) [PubMed] [Europe PMC] [Abstract]
  19. "Functional association of poly(ADP-ribose) polymerase with DNA polymerase alpha-primase complex: a link between DNA strand break detection and DNA replication."Nucleic Acids Res. 26:1891-1898(1998) [PubMed] [Europe PMC] [Abstract]
  20. "Suppression of the poly(ADP-ribose) polymerase activity by DNA-dependent protein kinase in vitro."Oncogene 18:4616-4625(1999) [PubMed] [Europe PMC] [Abstract]
  21. "Aprataxin, a novel protein that protects against genotoxic stress."Hum. Mol. Genet. 13:1081-1093(2004) [PubMed] [Europe PMC] [Abstract]
  22. "The poly(ADP-ribose) polymerase 1 interacts with Sry and modulates its biological functions."Mol. Cell. Endocrinol. 257:35-46(2006) [PubMed] [Europe PMC] [Abstract]
  23. "A novel human AP endonuclease with conserved zinc-finger-like motifs involved in DNA strand break responses."EMBO J. 26:2094-2103(2007) [PubMed] [Europe PMC] [Abstract]
  24. "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
  25. "Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins."Nature 451:81-85(2008) [PubMed] [Europe PMC] [Abstract]
  26. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  27. "Poly(ADP-ribose) polymerase-1 (PARP-1) transcriptionally regulates angiotensin AT2 receptor (AT2R) and AT2R binding protein (ATBP) genes."Biochem. Pharmacol. 77:1795-1805(2009) [PubMed] [Europe PMC] [Abstract]
  28. "PARP-1 transcriptional activity is regulated by sumoylation upon heat shock."EMBO J. 28:3534-3548(2009) [PubMed] [Europe PMC] [Abstract]
  29. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  30. "Poly(ADP-ribose)-dependent regulation of DNA repair by the chromatin remodeling enzyme ALC1."Science 325:1240-1243(2009) [PubMed] [Europe PMC] [Abstract]
  31. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  32. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  33. "Toward a unified nomenclature for mammalian ADP-ribosyltransferases."Trends Biochem. Sci. 35:208-219(2010) [PubMed] [Europe PMC] [Abstract]
  34. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  35. "Poly(ADP-ribose)-dependent regulation of Snail1 protein stability."Oncogene 30:4365-4372(2011) [PubMed] [Europe PMC] [Abstract]
  36. "Ubiquitin ligase RNF146 regulates tankyrase and Axin to promote Wnt signaling."PLoS ONE 6:E22595-E22595(2011) [PubMed] [Europe PMC] [Abstract]
  37. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  38. "Exome capture reveals ZNF423 and CEP164 mutations, linking renal ciliopathies to DNA damage response signaling." Cell 150:533-548(2012) [PubMed] [Europe PMC] [Abstract]
  39. "Systematic analysis of protein pools, isoforms, and modifications affecting turnover and subcellular localization."Mol. Cell. Proteomics 11:M111.013680.01-M111.013680.15(2012) [PubMed] [Europe PMC] [Abstract]
  40. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  41. "BAL1 and its partner E3 ligase, BBAP, link Poly(ADP-ribose) activation, ubiquitylation, and double-strand DNA repair independent of ATM, MDC1, and RNF8."Mol. Cell. Biol. 33:845-857(2013) [PubMed] [Europe PMC] [Abstract]
  42. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  43. "Solution structure of the first ZF-PARP domain, of the BRCT domain and of the WGR domain of human poly(ADP-ribose)polymerase-1."Submitted (APR-2007) to the PDB data bank
  44. "The consensus coding sequences of human breast and colorectal cancers." Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]