Wide-range ELISA Kit for Fatty Acid Binding Protein 1 (FABP1)
FABP-1; FABPL; L-FABP; LFABP; Liver-type fatty acid-binding protein; Fatty Acid Binding Protein 1, Liver
- Product No.WEB566Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range1.56-100ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.57ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Wide-range Fatty Acid Binding Protein 1 (FABP1).
No significant cross-reactivity or interference between Wide-range Fatty Acid Binding Protein 1 (FABP1) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Wide-range Fatty Acid Binding Protein 1 (FABP1) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range Fatty Acid Binding Protein 1 (FABP1) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Fatty Acid Binding Protein 1 (FABP1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Fatty Acid Binding Protein 1 (FABP1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range Fatty Acid Binding Protein 1 (FABP1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|J Korean Med Sci.||Acute Kidney Injury after Using Contrast during Cardiac Catheterization in Children with Heart Disease Pubmed:25120320|
|Cryobiology.?||Ex vivo use of a Rho-kinase inhibitor during renal preservation improves graft function upon reperfusion Pubmed:25555715|
|Clin Transl Sci||Controlled Rewarming after Hypothermia: Adding a New Principle to Renal Preservation PubMed: 26053383|
|SAGE journals||The role of serum I-FABP concentration in assessment of small intestine mucosa among HIV-infected patients Content: Early|
|Transplant International||Kidney transplantation after oxygenated machine perfusion preservation with Custodiol‐N solution PubMed: 25882869|
|European Journal of Clinical Investigation||Prediction of renal function upon reperfusion by ex situ controlled oxygenated rewarming pubmed:27718228|
|Journal of Clinical and Diagnostic Research||The Importance of Liver-Fatty Acid Binding Protein in Diagnosis of Liver Damage in Patients with Acute Hepatitis pubmed:28571184|
|Annals of Nutrition and Metabolism||Plasma Free Fatty Acids and their Binding Proteins in Preterm Infants Pubmed:30045009|
|Scandinavian Journal of Clinical and Laboratory Investigation||Isolated kidney perfusion: the influence of pulsatile flow Pubmed:29301417|