Wide-range ELISA Kit for Surfactant Associated Protein A (SPA)
PRL; SFTPA; PSAP; PSPA; SFTP; SFTPA1; COLEC4; PSP-A; SFTP1; SFTPA1B; SP-A1; Collectin-4; Alveolar proteinosis protein; 35 kDa pulmonary surfactant-associated protein
- Product No.WEA890Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range2.4-150ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.9ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 599
For more details, please contact local distributors! US$ 2696
For more details, please contact local distributors! US$ 5092
For more details, please contact local distributors! US$ 41930
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Wide-range Surfactant Associated Protein A (SPA).
No significant cross-reactivity or interference between Wide-range Surfactant Associated Protein A (SPA) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Wide-range Surfactant Associated Protein A (SPA) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range Surfactant Associated Protein A (SPA) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Surfactant Associated Protein A (SPA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Surfactant Associated Protein A (SPA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range Surfactant Associated Protein A (SPA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|The American Journal of Rhinology & Allergy||Surfactant protein A and D in chronic rhinosinusitis with nasal polyposis and corticosteroid response Ingenta: art00005|
|American Journal of Rhinology & Allergy||Detection of surfactant proteins A, B, C, and D in human nasal mucosa and their regulation in chronic rhinosinusitis with polyps PubMed: 23406594|
|Plos one||Staphylococcus aureus and Pseudomonas aeruginosa Express and Secrete Human Surfactant Proteins PubMed: PMC3551896|
|Plos one||The Detection of Surfactant Proteins A, B, C and D in the Human Brain and Their Regulation in Cerebral Infarction, Autoimmune Conditions and Infections of the CNS PubMed: PMC3787032|
|Nachweis und Charakterisierung des Oberfl?chenproteins PLUNC (Palate, Lung and Nasal Clone Protein) an der Augenoberfl?che und Bedeutung für das Trockene Auge Opus4:Source|
|Mol Med Rep||Expression of surfactant protein-A in exhaled breath condensate of patients with chronic obstructive pulmonary disease PubMed: 26707652|
|Plos One||Serum Levels of Surfactant Proteins in Patients with Combined Pulmonary Fibrosis and Emphysema (CPFE) Pubmed:27337142|
|PLoS One||The Cerebral Surfactant System and Its Alteration in HydrocephalicConditions. pubmed:27656877|
|Frontiers in Aging Neuroscience||Correlations of Ventricular Enlargement with Rheologically Active SurfactantProteins in Cerebrospinal Fluid. pubmed:28101052|
|Frontiers in Aging Neuroscience||Correlations of Ventricular Enlargement with Rheologically Active Surfactant Proteins in Cerebrospinal Fluid PMC5209370|
|American Journal of Physiology. Lung Cellular and Molecular Physiology||Chronic lung injury and impaired pulmonary function in a mouse model of acid ceramidase deficiency. pubmed:29167126|
|Molecular biosystems||Metabolic profiling of asthma in mice and the interventional effects of SPA using liquid chromatography and Q-TOF mass spectrometry pubmed:28463380|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA890Hu02||Recombinant Surfactant Associated Protein A (SPA)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA890Hu02||Polyclonal Antibody to Surfactant Associated Protein A (SPA)||WB; IHC; ICC; IP.|
|LAA890Hu81||FITC-Linked Polyclonal Antibody to Surfactant Associated Protein A (SPA)||WB; IHC; ICC; IF.|
|LAA890Hu71||Biotin-Linked Polyclonal Antibody to Surfactant Associated Protein A (SPA)||WB; IHC; ICC.|
|MAA890Hu22||Monoclonal Antibody to Surfactant Associated Protein A (SPA)||WB; IHC; ICC; IP.|
|WEA890Hu||Wide-range ELISA Kit for Surfactant Associated Protein A (SPA)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SEA890Hu||ELISA Kit for Surfactant Associated Protein A (SPA)||Enzyme-linked immunosorbent assay for Antigen Detection.|