ELISA Kit for Fibrinogen Like Protein 1 (FGL1)

FGL-1; HFREP1; HP-041; LFIRE1; Hepassocin; Hepatocyte-derived fibrinogen-related protein 1; Liver fibrinogen-related protein 1

Specificity

This assay has high sensitivity and excellent specificity for detection of Fibrinogen Like Protein 1 (FGL1).
No significant cross-reactivity or interference between Fibrinogen Like Protein 1 (FGL1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Fibrinogen Like Protein 1 (FGL1) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibrinogen Like Protein 1 (FGL1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-101 84
EDTA plasma(n=5) 84-92 88
heparin plasma(n=5) 83-92 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibrinogen Like Protein 1 (FGL1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibrinogen Like Protein 1 (FGL1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibrinogen Like Protein 1 (FGL1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-102% 78-104% 84-99% 82-99%
EDTA plasma(n=5) 90-101% 92-99% 79-99% 79-102%
heparin plasma(n=5) 81-102% 85-97% 80-94% 98-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
J Hepatol. The role of Hepassocin in the development of non-alcoholic fatty liver disease Pubmed: 23792031
Diabetes Management Serum hepassocin concentrations in diabetic patients with or without nonalcoholic fatty liver disease Futuremedicine:Source
United States Patent Application Compositions and Methods for Diagnosing Lung Cancer y2016:0077095.html
Cell Obesity Drives STAT-1-Dependent NASH and STAT-3-Dependent HCC Doi: 10.1016/j.cell.2018.09.053
Liver Research Do different bariatric surgery procedures impact hepassocin plasma levels in patients with type 2 diabetes mellitus?
Impacts of Different Modes of Bariatric Surgery on Plasma Levels of Hepassocin in Patients with Diabetes Mellitus
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPD022Ra01 Recombinant Fibrinogen Like Protein 1 (FGL1) Positive Control; Immunogen; SDS-PAGE; WB.
RPD022Ra02 Recombinant Fibrinogen Like Protein 1 (FGL1) Positive Control; Immunogen; SDS-PAGE; WB.
PAD022Ra01 Polyclonal Antibody to Fibrinogen Like Protein 1 (FGL1) WB; IHC; ICC; IP.
SED022Ra ELISA Kit for Fibrinogen Like Protein 1 (FGL1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD022Ra Multiplex Assay Kit for Fibrinogen Like Protein 1 (FGL1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSD022Ra01 ELISA Kit DIY Materials for Fibrinogen Like Protein 1 (FGL1) Main materials for "Do It (ELISA Kit) Yourself".