ELISA Kit for Glucose Transporter 4 (GLUT4)

SLC2A4; Solute carrier family 2, facilitated glucose transporter member 4; Glucose transporter type 4, insulin-responsive

Specificity

This assay has high sensitivity and excellent specificity for detection of Glucose Transporter 4 (GLUT4).
No significant cross-reactivity or interference between Glucose Transporter 4 (GLUT4) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucose Transporter 4 (GLUT4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucose Transporter 4 (GLUT4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Polar Record Glucose transporter-4 in white blood cells of young and old sled dogs: a model for human biomarker development Cambridge: Source
The International Journal of Biochemistry &amp; Cell Biology Conditioning causes an increase in glucose transporter-4 levels in mononuclear cells in sled dogs Pubmed:25236492
Psychoneuroendocrinology Maternal stress predicts altered biogenesis and the profile of mitochondrial proteins in the frontal cortex and hippocampus of adult offspring rats PubMed: 26143539
PLOS ONE Antidiabetic Effect of Galantamine: Novel Effect for a Known Centrally Acting Drug PubMed: 26262991
American Journal of Animal and Veterinary Sciences Metabolic Features of Heart Failure with Different Etiology ofsp11075
Journal of Pharmaceutical Negative Results Hibiscus sabdariffa linn fruits methanolic extract and fractions mediated glucose uptake stimulation and glucose transporter 4 regulation Doi: 10.4103/jpnr.JPNR_14_18
eureka herba indonesia Effect of Noni Fruit Extract (Morinda citrifolia) on Glucose Intake to Diabetes Mellitus White Rat Muscle Tissue
eureka herba indonesia The Effects of Sambiloto Leaf Extract (Andrographis peniculata) on Blood Sugar Regulation: an In Vivo Study
eureka herba indonesia Effect of Bay Leaf Extract (Syzygium polyanthum) on Blood Sugar Regulation via GLUT4 Protein Regulation in White Rat Muscle Tissue Induced Aloxan
Catalog No. Related products for research use of Canis familiaris; Canine (Dog) Organism species Applications (RESEARCH USE ONLY!)
RPC023Ca01 Recombinant Glucose Transporter 4 (GLUT4) Positive Control; Immunogen; SDS-PAGE; WB.
PAC023Ca01 Polyclonal Antibody to Glucose Transporter 4 (GLUT4) WB; IHC; ICC; IP.
MAC023Ca21 Monoclonal Antibody to Glucose Transporter 4 (GLUT4) WB; IHC; ICC; IP.
SEC023Ca ELISA Kit for Glucose Transporter 4 (GLUT4) Enzyme-linked immunosorbent assay for Antigen Detection.