ELISA Kit for Interleukin 31 (IL31)
- Product No.SEB179Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 6.1pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 684
For more details, please contact local distributors! US$ 3078
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For more details, please contact local distributors! US$ 47880
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Interleukin 31 (IL31).
No significant cross-reactivity or interference between Interleukin 31 (IL31) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Interleukin 31 (IL31) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 31 (IL31) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 31 (IL31) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 31 (IL31) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 31 (IL31) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|allergy and clinical immunology||Increased serum levels of Interleukin-31 could modulate inflammatory processes in multiple sclerosis Siaic-Review:Source|
|BMC Immunol||Increased levels of interleukin 31 (IL-31) in osteoporosis PubMed: 2644965|
|Sci Rep.||Interleukin-31 expression and relation to disease severity in human asthma pmc:PMC4783779|
|Exp Dermatol.||Immediate-type allergic and protease-mediated reactions are involved in scratching behaviour induced by topical application of Dermatophagoides farinae extract in NC/Nga mice. pubmed:28191683|
|Nat Commun.||The transcription factor EPAS1 links DOCK8 deficiency to atopic skin inflammation via IL-31 induction. pubmed:28067314|
|Experimental Dermatology||Immediate-type allergic and protease-media ted reactions are involved in scratching behavior induced by topical application of Dermatophagoides farinae extract in NC/Nga mice doi:10.1111|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|RPB179Ra01||Recombinant Interleukin 31 (IL31)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAB179Ra01||Polyclonal Antibody to Interleukin 31 (IL31)||WB; IHC; ICC; IP.|
|LAB179Ra81||FITC-Linked Polyclonal Antibody to Interleukin 31 (IL31)||WB; IHC; ICC; IF.|
|LAB179Ra71||Biotin-Linked Polyclonal Antibody to Interleukin 31 (IL31)||WB; IHC; ICC.|
|MAB179Ra21||Monoclonal Antibody to Interleukin 31 (IL31)||WB; IHC; ICC; IP.|
|SEB179Ra||ELISA Kit for Interleukin 31 (IL31)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSB179Ra01||ELISA Kit DIY Materials for Interleukin 31 (IL31)||Main materials for "Do It (ELISA Kit) Yourself".|