ELISA Kit for A Disintegrin And Metalloprotease 10 (ADAM10)

CD156-C; CD156c; MADM; Kuz; CDw156; Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease

Specificity

This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 10 (ADAM10).
No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 10 (ADAM10) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant A Disintegrin And Metalloprotease 10 (ADAM10) and the recovery rates were calculated by comparing the measured value to the expected amount of A Disintegrin And Metalloprotease 10 (ADAM10) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-94 86
EDTA plasma(n=5) 96-104 101
heparin plasma(n=5) 99-105 102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 10 (ADAM10) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 10 (ADAM10) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of A Disintegrin And Metalloprotease 10 (ADAM10) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-104% 85-94% 87-101% 81-104%
EDTA plasma(n=5) 78-89% 82-91% 87-94% 83-104%
heparin plasma(n=5) 96-103% 86-93% 86-103% 97-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Journal of Allergy and Clinical Immunology RAGE processing in chronic airway conditions: Involvement of Staphylococcus aureus and ECP ScienceDirect: S0091674912003508
British journal of cancer ADAM10: a new player in breast cancer progression? PubMed: 26284334
Int Wound J. From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. Pubmed:26991748
International Wound Journal From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases pubmed:26991748
Disease Markers Adamalysines as Biomarkers and a Potential Target of Therapy in Colorectal Cancer Patients: Preliminary Results Pubmed: 31565100
ADAM10 Plasma Levels Predict Worsening in Cognition of Older Adults: A 3-Year Follow-Up Study
Alzheimers Research & Therapy ADAM10 plasma levels predict worsening in cognition of older adults: a 3-year follow-up study. 33419480
life High Levels of TNF-¦Á and TIM-3 as a Biomarker of Immune Reconstitution Inflammatory Syndrome in People with HIV Infection
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