ELISA Kit for Toll Like Receptor 4 (TLR4)
CD284; TOLL; HToll
- Product No.SEA753Bo
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.625-40ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.281ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 756
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For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Toll Like Receptor 4 (TLR4).
No significant cross-reactivity or interference between Toll Like Receptor 4 (TLR4) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Toll Like Receptor 4 (TLR4) and the recovery rates were calculated by comparing the measured value to the expected amount of Toll Like Receptor 4 (TLR4) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Toll Like Receptor 4 (TLR4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Toll Like Receptor 4 (TLR4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Toll Like Receptor 4 (TLR4) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Chinese Medical Journal||Expression of soluble Toll-like receptors in pleural effusions CMJ: 2010823483999506297|
|Journal of Maternal-Fetal and Neonatal Medicine||Amniotic fluid soluble Toll-like receptor 4 in pregnancies complicated by preterm prelabor rupture of the membranes Informa: 14767058.2011.626821|
|PLoS One||Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway Inflammation PubMed: PMC3639905|
|Clin Res Hepatol Gastroenterol.||The association between precancerous gastric lesions and serum pepsinogens, serum gastrin, vascular endothelial growth factor, serum interleukin-1 Beta, serum toll-like receptor-4 levels and Helicobacter pylori Cag A status Pubmed: 23137754|
|Journal of Clinical Microbiology||Toll-Like Receptor 2 in Serum: a Potential Diagnostic Marker of Prosthetic Joint Infection? Pubmed: 24478497|
|Surgical Endoscopy||CO2 pneumoperitoneum increases secretory IgA levels in the gut compared with laparotomy in an experimental animal model. Pubmed: 24442682|
|Experimental and therapeutic medicine||Soluble toll-like receptor 4 reversed attenuating effect of Chinese herbal Xiao-Qing-Long-Tang on allergen induced nerve growth factor and thymic stromal lymphopoietin Pubmed: 24223644|
|Free Radical Biology and Medicine||Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis Pubmed:24992835|
|Respiratory Virology and Immunogenicity Advances in Experimental Medicine and Biology||Cytokines and Toll-Like Receptors in the Immune Response to Influenza Vaccination Pubmed:25248345|
|Molecular and Cellular Biochemistry||Beta-arrestin-2 negatively modulates inflammation response in mouse chondrocytes induced by 4-mer hyaluronan oligosaccharide Pubmed:25318610|
|Journal of Ginseng Research||Effects of Korean Red Ginseng (Panax ginseng), urushiol (Rhus vernicifera Stokes), and probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus acidophilus R0052) on the gut–liver axis of alcoholic liver disease Pubmed:25378990|
|BMC Immunology||The discriminative capacity of soluble Toll-like receptor (sTLR)2 and sTLR4 in inflammatory diseases Pubmed:25406630|
|Gastroenterology Research and Practice||Procoagulatory State in Inflammatory Bowel Diseases Is Promoted by Impaired Intestinal Barrier Function Pubmed:25767508|
|Eur J Gastroenterol Hepatol||Efficacy of rifaximin on circulating endotoxins and cytokines in patients with nonalcoholic fatty liver disease PubMed: 26043290|
|Pathol Biol (Paris)||Cathelicidin related antimicrobial peptide, laminin, Toll-like receptors and chemokines levels in experimental hypersensitivity pneumonitis in mice PubMed: 25834936|
|Journal of Food Science||Effects of Hyriopsis cumingii Polysaccharides on Mice Immunologic Receptor, Transcription Factor, and Cytokine Pubmed:27061742|
|Plos One||Soluble Toll-Like Receptors 2 and 4 in Cerebrospinal Fluid of Patients with Acute Hydrocephalus followingAneurysmal Subarachnoid Haemorrhage. Pubmed:27223696|
|Open Forum Infect Dis||The Effect of Rosuvastatin on Markers of Immune Activation in Treatment-Naive Human Immunodeficiency Virus-Patients Pubmed:26835476|
|Animal Feed Science and Technology||Impact of Saccharomyces cerevisiae fermentation product and subacute ruminal acidosis on production, inflammation, and fermentation in the rumen and hindCavia (Guinea pig )t of dairy cows science:S0377840115300493|
|european journal of pharmacology||Garlic active constituent s-allyl cysteine protects against lipopolysaccharide-induced cognitive deficits in the rat: Possible involved mechanisms. pubmed:27915041|
|european journal of pharmacology||S-allyl cysteine ameliorates cognitive deficits in streptozotocin-diabetic rats via suppression of oxidative stress, inflammation, and acetylcholinesterase. pubmed:27887948|
|Psychopharmacology (Berl)||Ellagic acid ameliorates learning and memory deficits in a rat model of Alzheimer's disease: an exploration of underlying mechanisms. pubmed:28303372|
|ЭНИ Забайкальский медицинский вестник||НОСИТЕЛЬСТВО ПОЛИМОРФИЗМА Thr399Ile и Asp299Gly ГЕНА TLR4 И УРОВЕНЬ TLR4 В ОПУХОЛЕВОЙ ТКАНИ У БОЛЬНЫХ ПРОЛИФЕРАТИВНЫМИ ЗАБОЛЕВАНИЯМИ МОЛОЧНОЙ ЖЕЛЕЗЫ 8.pdf|
|biomedicine & pharmacotherapy||Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat. pubmed:28199883|
|Journal For Neurovirology||Soluble TLR2 and 4 concentrations in cerebrospinal fluid in HIV/SIV-relatedneuropathological conditions. pubmed:27882497|
|Acta Orthopaedica||Toll-like receptors and their soluble forms differ in the knee and thumb basal osteoarthritic joints pubmed:28093922|
|Environmental Toxicology and Pharmacology||Ameliorative potential of linagliptin and/or calcipotriol on bleomycin-induced lung fibrosis: In vivo and in vitro study pubmed:28192751|
|Inflammation||Toll-Like Receptor 4 Inhibition Improves Oxidative Stress and Mitochondrial Health in Isoproterenol-Induced Cardiac Hypertrophy in Rats pubmed:28690610|
|Comparative Immunology, Microbiology and Infectious Diseases||Probiotic Lactobacillus acidophilus bacteria or synthetic TLR2 agonist boost the growth of chicken embryo intestinal organoids in cultures comprising epithelial cells and myofibroblasts DOI: 10.1016/j.cimid.2017.06.002|
|Comparative Immunology, Microbiology and Infectious Diseases||Probiotic Lactobacillus acidophilus bacteria or synthetic TLR2 agonist boost the growth of chicken embryo intestinal organoids in cultures comprising epithelial cells and myofibroblasts 10.1016/j.cimid.2017.06.002|
|Microbial Pathogenesis||Protective effects of sinomenine against LPS-induced inflammation in piglets. pubmed:28757275|
|Cytokine||Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effects pubmed:29102164|
|Biomedicine & pharmacotherapy||Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms pubmed:28061403|
|Frontiers in Immunology||The soluble Form of Toll-like receptor 2 is elevated in serum of Multiple sclerosis Patients: a novel Potential Disease Biomarker Pubmed:29593720|
|Experimental Gerontology||Specific alterations in the circulating levels of the SIRT1, TLR4, and IL7 proteins in patients with dementia Pubmed:30071285|
|Neurotoxicity Research||Protective Effects of Fibroblast Growth Factor 21 Against Amyloid-Beta-Induced Toxicity in SH-SY5Y Cells Pubmed:29869772|
|European Journal of Pharmacology||Rutin, a quercetin glycoside, alleviates acute endotoxemic kidney injury in C57BL/6 mice via suppression of inflammation and up-regulation of antioxidants and SIRT1 Pubmed:29920283|
|Environmental Toxicology and Pharmacology||Crocin reverses unilateral renal ischemia reperfusion injury-induced augmentation of oxidative stress and toll like receptor-4 activity Pubmed:29625388|
|Physiology & Behavior||Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll … Pubmed:29410018|
|International Journal of Gynecology & Obstetrics||Increased serum levels of high mobility group protein B1 and calprotectin in pre‐eclampsia Pubmed:29569400|
|Journal of Maternal-Fetal & Neonatal Medicine||Increased TLR4 and TREM-1 expression on monocytes and neutrophils in preterm birth: further evidence of a proinflammatory state Pubmed:29534643|
|ТLR4-РІВНІ У ХВОРИХ НА ПОДАГРУ: ЗВ'ЯЗОК З АКТИВНІСТЮ ЗАХВОРЮВАННЯ :|
|Journal of Medical Virology||Combined effect of anti‐high‐mobility group box‐1 monoclonal antibody and peramivir against influenza A virus‐induced pneumonia in mice Pubmed: 30281823|
|Metabolic Brain Disease||Berberine ameliorates lipopolysaccharide-induced learning and memory deficit in the rat: insights into underlying molecular mechanisms Pubmed: 30456649|
|Experimental and Therapeutic Medicine||The HMGB1‑RAGE/TLR‑TNF‑α signaling pathway may contribute to kidney injury induced by hypoxia|
|Immunopharmacology and immunotoxicology||Protective effect of sesamin in lipopolysaccharide-induced mouse model of acute kidney injury via attenuation of oxidative stress, inflammation, and apoptosis Pubmed: 30488751|
|International Immunopharmacology||S-allyl cysteine protects against lipopolysaccharide-induced acute kidney injury in the C57BL/6 mouse strain: Involvement of oxidative stress and inflammation Pubmed: 30665040|
|Biomedicine & Pharmacotherapy||Opuntioside, opuntiol and its metallic nanoparticles attenuate adjuvant-induced arthritis: Novel suppressors of Toll-like receptors-2 and-4|
|International Journal of Obesity||Circulating microbiota-derived metabolites: a “liquid biopsy? Pubmed: 31388096|
|Scientific Reports||Deregulation of TLR4 signaling pathway characterizes Bicuspid Aortic valve syndrome Pubmed: 31363123|
|Catalog No.||Related products for research use of Bos taurus; Bovine (Cattle) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA753Bo01||Recombinant Toll Like Receptor 4 (TLR4)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA753Bo01||Polyclonal Antibody to Toll Like Receptor 4 (TLR4)||WB; IHC; ICC; IP.|
|MAA753Bo21||Monoclonal Antibody to Toll Like Receptor 4 (TLR4)||WB; IHC; ICC; IP.|
|SEA753Bo||ELISA Kit for Toll Like Receptor 4 (TLR4)||Enzyme-linked immunosorbent assay for Antigen Detection.|