ELISA Kit for Prealbumin (PALB)
TBPA; PA; PLB; ATTR; TTR; Transthyretin; Prealbumin Amyloidosis Type I; Transports Thyroxine and Retinol
- Product No.SEA726Ca
- Organism SpeciesCanis familiaris; Canine (Dog) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range1.56-100ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.70ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 760
For more details, please contact local distributors! US$ 3420
For more details, please contact local distributors! US$ 6460
For more details, please contact local distributors! US$ 53200
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Prealbumin (PALB).
No significant cross-reactivity or interference between Prealbumin (PALB) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Prealbumin (PALB) and the recovery rates were calculated by comparing the measured value to the expected amount of Prealbumin (PALB) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prealbumin (PALB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prealbumin (PALB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Prealbumin (PALB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Chinese Medical Journal||Screening disease-associated proteins from sera of patients with rheumatoid arthritis: a comparative proteomic study PubMed: 20367977|
|Journal of Vascular Surgery||Circulation levels of acute phase proteins in patients with Takayasu arteritis PubMed: 20100644|
|Journal of Pharmaceutical and Biomedical Analysis||Effect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2 PubMed: 21130593|
|Toxicology Letters||Serum proteomic analysis reveals potential serum biomarkers for occupational medicamentosa-like dermatitis caused by trichloroethylene Pubmed:24960064|
|Journal of Neurochemistry||Schwann cells contribute to neurodegeneration in transthyretin amyloidosis Pubmed:25693163|
|Anal Biochem||Surface plasmon resonance assay of inhibition by pharmaceuticals for thyroxine hormone binging to transport proteins PubMed: 26384643|
|United States Patent Application||Compositions and Methods for Diagnosing Lung Cancer y2016:0077095.html|
|Neurotherapeutics||iTRAQ-Based Quantitative Proteomics Reveals the New Evidence Base for Traumatic Brain Injury Treated with Targeted Temperature Management pubmed:29247448|
|Neuroimmunomodulation||CD4+ T Cells Affect the Thyroid Hormone Transport at the Choroid Plexus in Mice Raised in Enriched Environment Pubmed: 30703773|
|Catalog No.||Related products for research use of Canis familiaris; Canine (Dog) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA726Ca01||Recombinant Prealbumin (PALB)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA726Ca01||Polyclonal Antibody to Prealbumin (PALB)||WB; IHC; ICC; IP.|
|SEA726Ca||ELISA Kit for Prealbumin (PALB)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSA726Ca01||ELISA Kit DIY Materials for Prealbumin (PALB)||Main materials for "Do It (ELISA Kit) Yourself".|