ELISA Kit for Prealbumin (PALB)

TBPA; PA; PLB; ATTR; TTR; Transthyretin; Prealbumin Amyloidosis Type I; Transports Thyroxine and Retinol


This assay has high sensitivity and excellent specificity for detection of Prealbumin (PALB).
No significant cross-reactivity or interference between Prealbumin (PALB) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Prealbumin (PALB) and the recovery rates were calculated by comparing the measured value to the expected amount of Prealbumin (PALB) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-101 89
EDTA plasma(n=5) 89-102 96
heparin plasma(n=5) 80-91 88


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prealbumin (PALB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prealbumin (PALB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Prealbumin (PALB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-104% 85-94% 78-94% 88-102%
EDTA plasma(n=5) 86-101% 91-105% 78-92% 97-104%
heparin plasma(n=5) 97-104% 78-95% 86-95% 89-99%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
Chinese Medical Journal Screening disease-associated proteins from sera of patients with rheumatoid arthritis: a comparative proteomic study PubMed: 20367977
Journal of Vascular Surgery Circulation levels of acute phase proteins in patients with Takayasu arteritis PubMed: 20100644
Journal of Pharmaceutical and Biomedical Analysis Effect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2 PubMed: 21130593
Toxicology Letters Serum proteomic analysis reveals potential serum biomarkers for occupational medicamentosa-like dermatitis caused by trichloroethylene Pubmed:24960064
Journal of Neurochemistry Schwann cells contribute to neurodegeneration in transthyretin amyloidosis Pubmed:25693163
Anal Biochem Surface plasmon resonance assay of inhibition by pharmaceuticals for thyroxine hormone binging to transport proteins PubMed: 26384643
United States Patent Application Compositions and Methods for Diagnosing Lung Cancer y2016:0077095.html
Neurotherapeutics iTRAQ-Based Quantitative Proteomics Reveals the New Evidence Base for Traumatic Brain Injury Treated with Targeted Temperature Management pubmed:29247448
Neuroimmunomodulation CD4+ T Cells Affect the Thyroid Hormone Transport at the Choroid Plexus in Mice Raised in Enriched Environment Pubmed: 30703773
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