ELISA Kit for Beta-2-Microglobulin (b2M)

BMG; B2MG

Specificity

This assay has high sensitivity and excellent specificity for detection of Beta-2-Microglobulin (b2M).
No significant cross-reactivity or interference between Beta-2-Microglobulin (b2M) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Beta-2-Microglobulin (b2M) and the recovery rates were calculated by comparing the measured value to the expected amount of Beta-2-Microglobulin (b2M) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-96 92
EDTA plasma(n=5) 95-102 99
heparin plasma(n=5) 98-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-2-Microglobulin (b2M) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-2-Microglobulin (b2M) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Beta-2-Microglobulin (b2M) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-94% 91-105% 93-104% 90-97%
EDTA plasma(n=5) 78-101% 78-93% 85-99% 89-96%
heparin plasma(n=5) 87-98% 95-105% 93-102% 85-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
BioMed Research International The Renal Effects of Vanadate Exposure: Potential Biomarkers and Oxidative Stress as a Mechanism of Functional Renal Disorders—Preliminary Studies Hindawi: 740105
BMC Nephrology Absence of chloride intracellular channel 4 (CLIC4) predisposes to acute kidney injury but has minimal impact on recovery Biomedcentral: Source
Nature Communications Mef2C restrains microglial inflammatory response and is lost in brain ageing in an IFN-I-dependent manner. pubmed:28959042
PLoS One Urine proteome analysis by C18 plate–matrix-assisted laser desorption/ionization time-of-flight mass spectrometry allows noninvasive differential diagnosis … Pubmed:30024955
Acta Anaesthesiologica Scandinavica Early prognostic factors in septic shock cancer patients: a prospective study with a proteomic approach Pubmed:29315472
Toxicology Mechanisms and Methods Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar rats Pubmed: 30426860
Journal of Clinical Medicine Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese Patients Pubmed: 30486327
Journal of Proteomics iTRAQ-based analysis for the identification of MARCH8 targets in human esophageal squamous cell carcinoma 33540066
Antioxidants Diminishment of Nrf2 Antioxidative Defense Aggravates Nephrotoxicity of Melamine and Oxalate Coexposure. Antioxidants 2021, 10, 1464 34573096
Int Arch Occup Environ Health Biomarkers of cadmium exposure and renal function in estuarine adult villagers 34773507
Catalog No. Related products for research use of Rhesus monkey (Simian) Organism species Applications (RESEARCH USE ONLY!)
RPA260Si01 Recombinant Beta-2-Microglobulin (b2M) Positive Control; Immunogen; SDS-PAGE; WB.
PAA260Si01 Polyclonal Antibody to Beta-2-Microglobulin (b2M) WB; IHC; ICC; IP.
SEA260Si ELISA Kit for Beta-2-Microglobulin (b2M) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA260Si Multiplex Assay Kit for Beta-2-Microglobulin (b2M) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.