ELISA Kit for Nerve Growth Factor (NGF)
NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor
- Product No.SEA105Po
- Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.4pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 756
For more details, please contact local distributors! US$ 3402
For more details, please contact local distributors! US$ 6426
For more details, please contact local distributors! US$ 52920
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Nerve Growth Factor (NGF).
No significant cross-reactivity or interference between Nerve Growth Factor (NGF) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Nerve Growth Factor (NGF) and the recovery rates were calculated by comparing the measured value to the expected amount of Nerve Growth Factor (NGF) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nerve Growth Factor (NGF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nerve Growth Factor (NGF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Nerve Growth Factor (NGF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|The Tohoku Journal of Experimental Medicine||Low-intensity aerobic exercise mitigates exercise-induced bronchoconstriction by improving the function of adrenal medullary chromaffin cells in asthmatic rats Pubmed:25212248|
|International Journal of Pharmaceutics||Neurotrophic and antioxidant effects of silymarin comparable to 4-methylcatechol in protection against gentamicin-induced ototoxicity in guinea pigs Pubmed:25445537|
|Yonsei Medical Journal||NGF and HB-EGF: Potential Biomarkers that Reflect the Effects of Fesoterodine in Patients with Overactive Bladder Syndrome Pubmed:25510766|
|Molecular and Cellular Endocrinology||High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 to increase NGF secretion in a rat Schwann cell line RSC96 Pubmed:25614971|
|Mol Cell Endocrinol||High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 PubMed: 25614971|
|Neurosciences (Riyadh)||Telmisartan inhibits hyperalgesia and inflammatory progression in a diabetic neuropathic pain model of Wistar rats. PubMed: 25864063|
|Neurotoxicity Research||Pro-apoptotic Action of Corticosterone in Hippocampal Organotypic Cultures Pubmed:27189478|
|Psychoneuroendocrinology||Chronic mild stress influences nerve growth factor through a matrix metalloproteinase-dependent mechanism Pubmed:26771945|
|Genet Mol Res||Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells Pubmed:27323064|
|The Journal of Maternal-Fetal & Neonatal Medicine||Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities pubmed:28081639|
|IOS Press Content Library||An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … articles:journal-of-alzheimers-disease|
|biblioteca.uva||Evaluación de la secreción de factores neurotróficos por las células MSV en cocultivo con neurorretinas porcinas, en función de la dosis celular y del tiempo de cultivo 10324/27440|
|Progress in Neuro-Psychopharmacology & Biological Psychiatry||Antidepressant drugs for beta amyloid-induced depression: A new standpoint? pubmed:28499898|
|Osteoarthritis and Cartilage||Mechanical loading of intervertebral disc modulates microglia proliferation, activation, and chemotaxis Pubmed:29723636|
|Stem Cell Research & Therapy||Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse model Pubmed:29335016|
|Pharmacological Research||N-3 PUFA diet enrichment prevents amyloid beta-induced depressive-like phenotype Pubmed:29203442|
|Experimental Neurology||Preterm umbilical cord blood derived mesenchymal stem/stromal cells protect preterm white matter brain development against hypoxia-ischemia Pubmed:30012511|
|Biochemical Pharmacology||Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in naïve and Aβ-treated female rats Pubmed:30028991|
|Experimental Dermatology||3‐‐Laurylglyceryl ascorbate improves the development of sensitive skin through the reduction of oxidative stress Pubmed: 30698879|
|BioMed Research International||Administration of Repetitive Transcranial Magnetic Stimulation Attenuates Aβ1-42-Induced Alzheimer's Disease in Mice by Activating β-Catenin Signaling|
|International Journal of Ophthalmology||A novel xeno-free culture system for human retinal pigment epithelium cells Pubmed: 31024807|
|Catalog No.||Related products for research use of Sus scrofa; Porcine (Pig) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA105Po01||Recombinant Nerve Growth Factor (NGF)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA105Po01||Polyclonal Antibody to Nerve Growth Factor (NGF)||WB; IHC; ICC; IP.|
|MAA105Po21||Monoclonal Antibody to Nerve Growth Factor (NGF)||WB; IHC; ICC; IP.|
|SEA105Po||ELISA Kit for Nerve Growth Factor (NGF)||Enzyme-linked immunosorbent assay for Antigen Detection.|