ELISA Kit for Matrix Metalloproteinase 3 (MMP3)
SL1; STMY; STMY1; STR1; Progelatinase; Stromelysin 1; Transin-1
- Product No.SEA101Po
- Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
- Sample Typeserum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range4.69-300ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 1.8ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 3 (MMP3).
No significant cross-reactivity or interference between Matrix Metalloproteinase 3 (MMP3) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Matrix Metalloproteinase 3 (MMP3) and the recovery rates were calculated by comparing the measured value to the expected amount of Matrix Metalloproteinase 3 (MMP3) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 3 (MMP3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 3 (MMP3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Matrix Metalloproteinase 3 (MMP3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Journal of Bone and Joint Surgery||Experimental osteoarthritis induced by surgical realignment of the patella in BALB/c mice PubMed: 21119180|
|Infection and Immunity||Bovine Respiratory Syncytial Virus and Histophilus somni Interaction at the Alveolar Barrier Pubmed: 23649093|
|PLoS ONE||A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source|
|China Pharmacy||Influence of Tongfengtai Granules on Inflammatory Factor in Acute Gouty Arthritis Model Rats Source|
|Thromb Haemost.||Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke Pubmed:24671655|
|Connective Tissue Research||Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes Pubmed:25333747|
|physiological research||The skin remodeling in type 1 diabetes and insulin resistance animal models PubMed: 26047379|
|Physiol Res||The skin remodeling in type 1 diabetes and insulin resistance animal models. PubMed: 26047379|
|Cellular & Molecular Immunology||Effect of ebosin on modulating interleukin-2&bgr;-induced inflammatory responses in rat fibroblast-like synoviocytes PubMed: 25938977|
|Biol Res||The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis PubMed: 26578310|
|J Orthop Res.||Tetrandrine suppresses articular inflammatory response by inhibiting pro-inflammatory factors via NF-κB inactivation. Pubmed:26748661|
|Cellular & Molecular Immunology||Effect of ebosin on modulating interleukin-1β-induced inflammatory responses in rat fibroblast-like synoviocytes pubmed:25938977|
|Journal of Orthopaedic Research||Tetrandrine suppresses articular inflammatory response by inhibiting pro‐inflammatory factors via NF‐κB inactivation pubmed:26748661|
|Mediators of Inflammation||Interleukin-29 Enhances Synovial Inflammation and Cartilage Degradation in Osteoarthritis. pubmed:27433031|
|Molecular Medicine Reports||Icariin inhibits MMP‑1, MMP‑3 and MMP‑13 expression through MAPK pathways in IL‑1β‑stimulated SW1353 chondrosarcoma cells pubmed:28447732|
|der Christian-Albrechts-Universität zu Kiel||Wirkung schonender Laserverfahren auf den Komplexaus retinalem Pigmentepithel, Bruch´scher Membranund Choroidea - mögliche Therapieoption der frühen altersabhängigen Makuladegeneration 1133074014:34|
|REDVET - Revista electrónica de Veterinaria||Measurement of Metalloproteinase-3 levels in synovial fluid from horses as biomarker of joint disease E-ISSN: 1695-7504|
|REDVET - Revista electrónica de Veterinaria||Measurement of Metalloproteinase-3 levels in synovial fluid from horses as biomarker of joint disease - Medición de los niveles de Metaloproteinasa-3 en liquido sinovial comobiomarcador de enfermedad articular ISSN 1695-7504|
|Molecular Pharmacology||Dexamethasone Down-regulates Endothelin Receptors and Reduces Endothelin-induced Production of Matrix Metalloproteinases in Cultured Rat Astrocytes 10.1124/mol.116.107300|
|Catalog No.||Related products for research use of Sus scrofa; Porcine (Pig) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA101Po01||Recombinant Matrix Metalloproteinase 3 (MMP3)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA101Po01||Polyclonal Antibody to Matrix Metalloproteinase 3 (MMP3)||WB; IHC; ICC; IP.|
|MAA101Po21||Monoclonal Antibody to Matrix Metalloproteinase 3 (MMP3)||WB; IHC; ICC; IP.|
|SEA101Po||ELISA Kit for Matrix Metalloproteinase 3 (MMP3)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA101Po||CLIA Kit for Matrix Metalloproteinase 3 (MMP3)||Chemiluminescent immunoassay for Antigen Detection.|