ELISA Kit for Leptin (LEP)
OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein
- Product No.SEA084Ov
- Organism SpeciesOvis aries; Ovine (Sheep) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.054ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 810
For more details, please contact local distributors! US$ 3645
For more details, please contact local distributors! US$ 6885
For more details, please contact local distributors! US$ 56700
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Leptin (LEP).
No significant cross-reactivity or interference between Leptin (LEP) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Leptin (LEP) and the recovery rates were calculated by comparing the measured value to the expected amount of Leptin (LEP) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leptin (LEP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leptin (LEP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Leptin (LEP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|European Journal of Pharmacology||Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet PubMed: 20869961|
|Indian Journal of Pharmacology||Effect of Argyreia speciosa root extract on cafeteria diet-induced obesity in rats Ijp: source|
|Journal of endocrinology||Testosterone protects from metabolic syndrome-associated prostate inflammation: an experimental study in rabbit PubMed: 22010203|
|PLoS One.||Multi-Functional Roles of Chitosan as a Potential Protective Agent against Obesity PubMed: 23342013|
|Molecular and Cellular Endocrinology||Soy isoflavones modulate adipokines and myokines to regulate lipid metabolism in adipose tissue, skeletal muscle and liver of male Huanjiang mini-pigs ScienceDirect: S0303720712004194|
|Journal of Animal and Feed Sciences||Ovarian, metabolic and endocrine indexes in dairy cows with different body condition scores Ifzz: Source|
|British Journal of Nutrition||n-6: n-3 PUFA ratio is involved in regulating lipid metabolism and inflammation in pigs Pubmed: 23947577|
|Int J Mol Sci.||Maternal high folic acid supplement promotes glucose intolerance and insulin resistance in male mouse offspring fed a high-fat diet. Pubmed: 24736781|
|Endocr J.||CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes Pubmed:25168658|
|Animal Physiology||EFFECT OF LEPTIN GENE ON SERUM LEPTIN CONCENTRATION AND BEEF QUALITY IN CZECH FLECKVIEH BULLS Mendelu:Source|
|Evid Based Complement Alternat Med.||Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar Rats Pubmed:Pmc4363645|
|general endocrinology||Oxytocin reverse ovariectomy-induced Osteoper and body fat gain Pubmed:24506069|
|Respiratory Physiology & Neurobiology||Maternal high-fat diet feeding during pregnancy and lactation augments lung inflammation and remodeling in the offspring Pubmed:25500158|
|Sci Rep||Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis PubMed: 26634430|
|Evid Based Complement Alternat Med||Tinospora crispa PubMed: 25821506|
|Ankara||Effects of breeder age and early energy restriction on fattening performance, some meat quality traits and plasma leptin concentration of broilers Dergiler: 11|
|MEDICINA VETERIN||Comparative measurements of the morphometric pattern and energy profile of Crioula mares in the final third of gestation, with different body condition scores Scielo: S0102-09352015000300707|
|Pancreatic Disorders & Therapy||Pancreatic Exocrine Insufficiency Affects Not Only Digestibility of Nutrients and Growth, but Also Body Composition and Endocrinological Parameters – Study on Piglets Used As a Model for Children Journals: 2165-7092-%20S5-00|
|Veterinary Word||Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers Pubmed:27051212|
|Animal||Anterior pituitary influence on adipokine expression and secretion by porcine adipocytes. Pubmed:27074715|
|ANNALS OF ANIMAL SCIENCE||Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens journalprintahead:87|
|Annals of Animal Science||Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens view:87|
|Obes Surg.||Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in Obese Göttingen Minipig. Effects on Glucose Metabolism. Pubmed:27185175|
|Acta Histochemica||Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines science:S0065128116301234|
|J Biomed Mater Res A||Nanosized titanium dioxide resulted in the activation of TGF-β/Smads/p38MAPK pathway in renal inflammation and fibration of mice Pubmed:26850371|
|Clin Epigenetics.||The bromodomain inhibitor N-methyl pyrrolidone reduced fat accumulation in an ovariectomized rat model Pubmed:27110299|
|International Journal of Molecular Sciences||Oral Supplementation with a Special Additive of Retinyl Palmitate and Alpha Tocopherol Reduces Growth Retardation in Young Pancreatic Duct Ligated Pigs Used as a Model for Children Suffering from Exocrine Pancreatic Insufficiency pubmed:27690005|
|Journal of Animal Science||Changes in small intestinal morphometry and electrogenic nutrient transport in young pigs with pancreatic exocrine insufficiency publications:jas|
|International Journal of Endocrinology||The Changes of Serum Leptin and Kisspeptin Levels in Chinese Children and Adolescents in Different Pubertal Stages pubmed:27990162|
|journal of animal science||The effect of GnRH vaccination on performance, carcass, and meat quality and hormonal regulation in boars, barrows, and gilts. pubmed:27482668|
|journal of animal science||Diet-induced modifications to milk composition have long-term effects on offspring growth in rabbits. pubmed:28380600|
|Evidence-Based Complementary and Alternative Medicine||Four-Week Consumption of Malaysian Honey Reduces Excess Weight Gainand Improves Obesity-Related Parameters in High Fat Diet Induced ObeseRats. pubmed:28246535|
|Asian-Australasian Journal of Animal Sciences||Dietary Niacin Supplementation Suppressed Hepatic Lipid Accumulation in Rabbits pubmed:27004817|
|Stem Cell Research & Therapy||Serial changes in the proliferation and differentiation of adipose-derived stem cells after ionizingradiation. pubmed:27530249|
|Obesity Surgery||Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in ObeseGöttingen Minipig. Effects on Glucose Metabolism. pubmed:27185175|
|Meat Sci||Estimation of the intramuscular fat content of m. longissimus thoracis in crossbred beef cattle based on live animal measurements. pubmed:27940421|
|THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY||Correlation of Leptin, Insulin and Glucose During Late Gestation and Early Lactation in Murrah Buffaloes 7086|
|Journal of Animal and Plant Sciences||Effects Of Maca (Lepıdıum Meyenıı Walp) Powder On Serum Indıces And Metabolıc Responses In Racehorses 57cf073008ae83b37462323a.pdf|
|Biological Trace Element Research||Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Diet article:10.1007|
|Revista Brasileira de Ciência Avícola||Effect of Zinc Level and Source (Zinc Oxide Vs. Zinc Glycine) on Bone Mechanical and Geometric Parameters, and Histomorphology in Male Ross 308 Broiler Chicken 10.159:1806-9061-2016-0285|
|Applied Animal Behaviour Science||Evaluating measures of exploratory behaviour in sows around farrowing and during lactation− A pilot study 10.1016/j.jacl.2017.03.007|
|Proceedings of the National Academy of Sciences of the United States of America (PNAS)||cAMP-inducible coactivator CRTC3 attenuates brown adipose tissue thermogenesis Pubmed:29784793|
|Oxidative Medicine and Cellular Longevity||Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague … Pubmed:29849871|
|Frontiers in Endocrinology||Circulating and Adipose Tissue mRNA Levels of Zinc-α2-Glycoprotein, Leptin, High-Molecular-Weight Adiponectin, and Tumor Necrosis Factor-Alpha in … Pubmed:29755407|
|Journal of Agricultural and Food Chemistry||Metabolomics for the effect of biotin and nicotinamide on transition dairy cows Pubmed:29758980|
|Frontiers in Physiology||Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21 Pubmed:29593555|
|Bioscience Reports||Effect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal rats Pubmed:29784871|
|Neurochemical Research||The leptin, dopamine and serotonin receptors in hypothalamic POMC-neurons of normal and obese rodents Pubmed:29397535|
|PLoS One||Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickens Pubmed:29373588|
|Biochemical and Biophysical Research Communications||Chronic cold exposure results in subcutaneous adipose tissue browning and altered global metabolism in Qinghai-Tibetan plateau pika (Ochotona curzoniae) Pubmed:29626477|
|Alcohol and Alcoholism||Leptin Expression and Gene Methylation Patterns in Alcohol-Dependent Patients with Ethyltoxic Cirrhosis—Normalization After Liver Transplantation and Implications … Pubmed:29912265|
|Iranian Red Crescent Medical Journal||The Effect of Aerobic Training on Tumor Growth, Adiponectin, Leptin and Ghrelin in Mice with Breast Cancer ircmj-20-03-13305.pdf|
|Pakistan Journal of Zoology||Effects of Intrauterine Growth Retardation on Growth, Meat Quality and Muscle Fiber Composition of Pigs. :|
|Oncotarget||Epigallocatechin-3-gallate suppresses differentiation of adipocytes via regulating the phosphorylation of FOXO1 mediated by PI3K-AKT signaling in 3T3-L1 … Pubmed:29484120|
|УСПЕХИ ГЕРОНТОЛОГИИ||ВЛИЯНИЕ МЕТФОРМИНА НА МЕТАБОЛИЧЕСКИЕ ПОКАЗАТЕЛИ И ГИПОТАЛАМИЧЕСКИЕ СИГНАЛЬНЫЕ СИСТЕМЫ У КРЫС С ОЖИРЕНИЕМ … AG_2018-31-01.pdf|
|Catalog No.||Related products for research use of Ovis aries; Ovine (Sheep) Organism species||Applications (RESEARCH USE ONLY!)|
|SEA084Ov||ELISA Kit for Leptin (LEP)||Enzyme-linked immunosorbent assay for Antigen Detection.|