ELISA Kit for E-selectin
CD62E; CD62-E; E-Selectin; ELAM; ELAM1; ESEL; SEL-E; E-LAM; E-LAM1; E-SEL; LECAM2; Endothelial Leukocyte Adhesion Molecule 1; CD62 Antigen-Like Family Member E
- Product No.SEA029Ca
- Organism SpeciesCanis familiaris; Canine (Dog) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range6.25-400pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 2.84pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 720
For more details, please contact local distributors! US$ 3240
For more details, please contact local distributors! US$ 6120
For more details, please contact local distributors! US$ 50400
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of E-selectin.
No significant cross-reactivity or interference between E-selectin and analogues was observed.
Matrices listed below were spiked with certain level of recombinant E-selectin and the recovery rates were calculated by comparing the measured value to the expected amount of E-selectin in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level E-selectin were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level E-selectin were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E-selectin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Shock||Systemic involvement of high-mobility group box 1 protein and therapeutic effect of anti-high-mobility group box 1 protein antibody in a rat model of crush injury. PubMed: 22392147|
|BioMed research international||Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations Pubmed: 24199192|
|Journal of Neuroimmunology||Evaluation of the effects of Eserine and JWH-133 on brain dysfunction associated with experimental endotoxemia PubMed: 25867462|
|International Journal of Food Sciences and Nutrition||Serum lipid profile and inflammatory markers in the aorta of cholesterol-fed rats supplemented with extra virgin olive oil, sunflower oils and oil-products PubMed: 26401576|
|Experimental Parasitology||Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis pubmed:28821422|
|Catalog No.||Related products for research use of Canis familiaris; Canine (Dog) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA029Ca01||Recombinant E-selectin||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA029Ca01||Polyclonal Antibody to E-selectin||WB; IHC; ICC; IP.|
|MAA029Ca21||Monoclonal Antibody to E-selectin||WB; IHC; ICC; IP.|
|SEA029Ca||ELISA Kit for E-selectin||Enzyme-linked immunosorbent assay for Antigen Detection.|