CLIA Kit for Growth Differentiation Factor 11 (GDF11)

BMP11; Bone morphogenetic protein 11


This assay has high sensitivity and excellent specificity for detection of Growth Differentiation Factor 11 (GDF11).
No significant cross-reactivity or interference between Growth Differentiation Factor 11 (GDF11) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Growth Differentiation Factor 11 (GDF11) and the recovery rates were calculated by comparing the measured value to the expected amount of Growth Differentiation Factor 11 (GDF11) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-105 101
EDTA plasma(n=5) 95-104 101
heparin plasma(n=5) 92-102 99


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Differentiation Factor 11 (GDF11) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Differentiation Factor 11 (GDF11) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Growth Differentiation Factor 11 (GDF11) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-101% 97-104% 80-104% 79-94%
EDTA plasma(n=5) 82-99% 93-103% 83-102% 96-103%
heparin plasma(n=5) 79-94% 90-101% 81-89% 91-98%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.



Magazine Citations
Oncotarget Splenocytes derived from young WT mice prevent AD progression in APPswe/PSENldE9 transgenic mice PubMed: 26317549
Endocrinology TGFβ Superfamily Members Mediate Androgen Deprivation Therapy-Induced Obese Frailty in Male Mice pubmed:27611336
International Journal of Clinical and Experimental Pathology GDF11 is increased in patients with myelodysplastic syndrome files:ijcep0024948.pdf
European Review for Medical and Pharmacological Sciences GDF11 does not improve the palmitate induced insulin resistance in C2C12. pubmed:28485800
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