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Polyclonal Antibody to Mdm2 p53 Binding Protein Homolog (MDM2)

HDMX; hdm2; Mouse Double Minute 2,Human Homolog Of p53-Binding Protein; Oncoprotein Mdm2

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against MDM2. It has been selected for its ability to recognize MDM2 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Clin Cancer Res Inactivation of the p53–KLF4–CEBPA Axis in Acute Myeloid Leukemia PubMed: 26408402
Clin Cancer Res Inactivation of the p53–KLF6–CEBPA Axis in Acute Myeloid Leukemia PubMed: 26408402
Analyst Sensitive and simultaneous surface plasmon resonance detection of free and p53-bound MDM2 proteins from human sarcomas Pubmed:29637949
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