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Mini Samples ELISA Kit for Interleukin 28B (IL28B)
IFNL3; Interferon,Lambda 3; Cytokine Zcyto22
- Product No.MEC028Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 6.2pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 605
US$ 864
US$ 3888
US$ 7344
US$ 60480
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 28B (IL28B).
No significant cross-reactivity or interference between Mini Samples Interleukin 28B (IL28B) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Mini Samples Interleukin 28B (IL28B) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Interleukin 28B (IL28B) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 88-99 | 93 |
| EDTA plasma(n=5) | 86-101 | 95 |
| heparin plasma(n=5) | 94-105 | 97 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 28B (IL28B) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 28B (IL28B) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Interleukin 28B (IL28B) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 99-105% | 78-101% | 97-105% | 91-98% |
| EDTA plasma(n=5) | 97-105% | 83-90% | 84-104% | 96-105% |
| heparin plasma(n=5) | 80-89% | 99-105% | 91-105% | 87-98% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×60µL | Assay Diluent A | 1×6mL |
| Detection Reagent B | 1×60µL | Assay Diluent B | 1×6mL |
| TMB Substrate | 1×4.5mL | Stop Solution | 1×3mL |
| Wash Buffer (30 × concentrate) | 1×10mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.
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| Magazine | Citations |
| Journal of Antimicrobial Chemotherapy | Impact of IL28B gene polymorphisms on interferon-λ3 plasma levels during pegylated interferon-α/ribavirin therapy for chronic hepatitis C in patients coinfected with HIV PubMed: 22294646 |
| Cytokine | Different effects of three interferons L on Toll-like receptor-related gene expression in HepG2 cells. Pubmed: 24041672 |
| Hepatology | Polymorphisms in melanoma differentiation‐associated gene 5 link protein function to clearance of hepatitis C virus Pubmed:25130193 |
| Cytokine. | Increased concentration of interferon lambda-3, interferon beta and interleukin-10 in the cerebrospinal fluid of patients with tick-borne encephalitis Pubmed:25461389 |
| J Biomed Res. | Impact of IL28B gene polymorphisms rs8099917 and rs12980275 on response to pegylated interferon-α/ribavirin therapy in chronic hepatitis C genotype 4 … pmc:PMC4726832 |
| Journal of Biomedical Research | Impact of IL28B gene polymorphisms rs8099917 and rs12980275 on response to pegylated interferon-α/ribavirin therapy in chronic hepatitis C genotype 4 patients PMC4726832 |
| LUPUS | Collagen triple helix repeat containing-1: a novel biomarker associated with disease activity in Systemic lupus erythematosus Pubmed: 30336754 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| MEC028Mu | Mini Samples ELISA Kit for Interleukin 28B (IL28B) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC028Mu | Multiplex Assay Kit for Interleukin 28B (IL28B) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |