ELISA Kit DIY Materials for Wingless Type MMTV Integration Site Family, Member 3A (WNT3A)

  • Product No.KSP155Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.061ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1368  US$ 2280  US$ 4104  US$ 7980  US$ 13680 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 3A (WNT3A). No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Medical Oncology Wls promotes the proliferation of breast cancer cells via Wnt signaling Pubmed:25801232
J Histochem Cytochem Wnt3a Mediates the Inhibitory Effect of Hyperoxia on the Transdifferentiation of AECIIs to AECIs PubMed: 26209081
PloS one Gelatin device for the delivery of growth factors involved in endochondral ossification pubmed:28380024
Life Sciences Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways. pubmed:28754618
Oxidative Medicine and Cellular Longevity Secreted Frizzled-Related Protein-2 Inhibits Doxorubicin Induced Apoptosis Mediated Through the Akt-mTOR Pathway in Soleus Muscle Pubmed:30151071
The Showa University Journal of Medical Sciences Eribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β-catenin Signaling Pathway in Triple-negative Breast Cancer Doi: 10.15369/sujms.30.359
Journal of Cancer Oncogenic Wnt3a: A Candidate Specific Marker and Novel Molecular Target for Hepatocellular Carcinoma Pubmed: 31737122
CANCER LETTERS Knock down of BMSC-derived Wnt3a or its antagonist analogs attenuate colorectal carcinogenesis induced by chronic Fusobacterium nucleatum infection Pubmed: 32920199
Biomaterials An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties 34823885
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPP155Ra01 Recombinant Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) Positive Control; Immunogen; SDS-PAGE; WB.
PAP155Ra01 Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) WB; IHC; ICC; IP.
MAP155Ra21 Monoclonal Antibody to Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) WB; IHC; ICC; IP.
SEP155Ra ELISA Kit for Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) Enzyme-linked immunosorbent assay for Antigen Detection.
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LMP155Ra Multiplex Assay Kit for Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSP155Ra01 ELISA Kit DIY Materials for Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) Main materials for "Do It (ELISA Kit) Yourself".