ELISA Kit DIY Materials for Fibrinogen Like Protein 1 (FGL1)

FGL-1; HFREP1; HP-041; LFIRE1; Hepassocin; Hepatocyte-derived fibrinogen-related protein 1; Liver fibrinogen-related protein 1

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Fibrinogen Like Protein 1 (FGL1).No significant cross-reactivity or interference between Fibrinogen Like Protein 1 (FGL1) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
J Hepatol. The role of Hepassocin in the development of non-alcoholic fatty liver disease Pubmed: 23792031
Diabetes Management Serum hepassocin concentrations in diabetic patients with or without nonalcoholic fatty liver disease Futuremedicine:Source
United States Patent Application Compositions and Methods for Diagnosing Lung Cancer y2016:0077095.html
Cell Obesity Drives STAT-1-Dependent NASH and STAT-3-Dependent HCC Doi: 10.1016/j.cell.2018.09.053
Liver Research Do different bariatric surgery procedures impact hepassocin plasma levels in patients with type 2 diabetes mellitus?
Impacts of Different Modes of Bariatric Surgery on Plasma Levels of Hepassocin in Patients with Diabetes Mellitus
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