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ELISA Kit DIY Materials for Vimentin (VIM)

  • Product No.KSB040Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range125-8000pg/mL
  • Applicable Sensitivity46pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1197  US$ 1995  US$ 3591  US$ 6983  US$ 11970 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Vimentin (VIM). No significant cross-reactivity or interference between Vimentin (VIM) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Cancer Epidemiology, Biomarkers & Prevention Aberrant Vimentin Methylation Is Characteristic of Breast Cancer pubmed:22315367
Scientific Reports Glyoxalase 1-knockdown in human aortic endothelial cells - effect on the proteome andendothelial function estimates. pubmed:27898103
Journal of Proteomics HtrA3 is a cellular partner of cytoskeleton proteins and TCP1α chaperonin Pubmed:29477555
Journal of Biological Chemistry Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy Pubmed: 31511328
American Journal of Transplantation Extracellular vesicles derived from injured vascular tissue promote the formation of tertiary lymphoid structures in vascular allografts Pubmed: 31729155
Lung Is Vimentin the Cause or Effect of Obstructive Sleep Apnea Development? Pubmed: 32088750
Cell Biol Toxicol ZNF471 modulates EMT and functions as methylation regulated tumor suppressor with diagnostic and prognostic significance in cervical cancer 33566221
CELL BIOLOGY AND TOXICOLOGY Metastatic suppression by DOC2B is mediated by inhibition of epithelial-mesenchymal transition and induction of senescence 33758996
Comparison of Vimentin Levels Between Preeclamptic and Normotensive Pregnant Women
Advanced glycation end-products associate with podocytopathy in type II diabetic patients
Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β …
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB040Hu02 Recombinant Vimentin (VIM) Positive Control; Immunogen; SDS-PAGE; WB.
APB040Hu01 Active Vimentin (VIM) Cell culture; Activity Assays.
RPB040Hu01 Recombinant Vimentin (VIM) Positive Control; Immunogen; SDS-PAGE; WB.
PAB040Hu02 Polyclonal Antibody to Vimentin (VIM) WB; IHC; ICC; IP.
PAB040Hu01 Polyclonal Antibody to Vimentin (VIM) WB; IHC; ICC/IF
LAB040Hu71 Biotin-Linked Polyclonal Antibody to Vimentin (VIM) WB; IHC; ICC.
MAB040Hu24 Monoclonal Antibody to Vimentin (VIM) WB; IHC
MAB040Hu25 Monoclonal Antibody to Vimentin (VIM) WB; IHC; IF
MAB040Hu21 Monoclonal Antibody to Vimentin (VIM) WB; IHC; ICC; IP.
MAB040Hu23 Monoclonal Antibody to Vimentin (VIM) WB; IHC; ICC; IP.
MAB040Hu27 Monoclonal Antibody to Vimentin (VIM) WB; IHC; ICC; IP.
MAB040Hu22 Monoclonal Antibody to Vimentin (VIM) WB; IHC; IF
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FAB040Hu04 Anti-Vimentin (VIM) Monoclonal Antibody Flow cytometry.
FAB040Hu08 Anti-Vimentin (VIM) Monoclonal Antibody Flow cytometry.
SEB040Hu ELISA Kit for Vimentin (VIM) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB040Hu Multiplex Assay Kit for Vimentin (VIM) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB040Hu01 ELISA Kit DIY Materials for Vimentin (VIM) Main materials for "Do It (ELISA Kit) Yourself".