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Active Vimentin (VIM)

ACTIVITY TEST

Vimentin (VIM) is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells.vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression. Besides, Keratin 20 (KRT20) has been identified as an interactor of VIM, thus a binding ELISA assay was conducted to detect the interaction of recombinant human VIM and recombinant human KRT20. Briefly, VIM were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl then transferred to KRT20-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-VIM pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of VIM and KRT20 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Cancer Epidemiology, Biomarkers & Prevention Aberrant Vimentin Methylation Is Characteristic of Breast Cancer pubmed:22315367
Scientific Reports Glyoxalase 1-knockdown in human aortic endothelial cells - effect on the proteome andendothelial function estimates. pubmed:27898103
Journal of Proteomics HtrA3 is a cellular partner of cytoskeleton proteins and TCP1α chaperonin Pubmed:29477555
Journal of Biological Chemistry Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy Pubmed: 31511328
American Journal of Transplantation Extracellular vesicles derived from injured vascular tissue promote the formation of tertiary lymphoid structures in vascular allografts Pubmed: 31729155
Lung Is Vimentin the Cause or Effect of Obstructive Sleep Apnea Development? Pubmed: 32088750
Cell Biol Toxicol ZNF471 modulates EMT and functions as methylation regulated tumor suppressor with diagnostic and prognostic significance in cervical cancer 33566221
CELL BIOLOGY AND TOXICOLOGY Metastatic suppression by DOC2B is mediated by inhibition of epithelial-mesenchymal transition and induction of senescence 33758996
Comparison of Vimentin Levels Between Preeclamptic and Normotensive Pregnant Women
Advanced glycation end-products associate with podocytopathy in type II diabetic patients
Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β …
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB040Hu02 Recombinant Vimentin (VIM) Positive Control; Immunogen; SDS-PAGE; WB.
APB040Hu01 Active Vimentin (VIM) Cell culture; Activity Assays.
RPB040Hu01 Recombinant Vimentin (VIM) Positive Control; Immunogen; SDS-PAGE; WB.
PAB040Hu02 Polyclonal Antibody to Vimentin (VIM) WB; IHC; ICC; IP.
PAB040Hu01 Polyclonal Antibody to Vimentin (VIM) WB; IHC; ICC/IF
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MAB040Hu24 Monoclonal Antibody to Vimentin (VIM) WB; IHC
MAB040Hu25 Monoclonal Antibody to Vimentin (VIM) WB; IHC; IF
MAB040Hu21 Monoclonal Antibody to Vimentin (VIM) WB; IHC; ICC; IP.
MAB040Hu23 Monoclonal Antibody to Vimentin (VIM) WB; IHC; ICC; IP.
MAB040Hu27 Monoclonal Antibody to Vimentin (VIM) WB; IHC; ICC; IP.
MAB040Hu22 Monoclonal Antibody to Vimentin (VIM) WB; IHC; IF
FAB040Hu02 Anti-Vimentin (VIM) Monoclonal Antibody Flow cytometry.
FAB040Hu06 Anti-Vimentin (VIM) Monoclonal Antibody Flow cytometry.
FAB040Hu04 Anti-Vimentin (VIM) Monoclonal Antibody Flow cytometry.
FAB040Hu08 Anti-Vimentin (VIM) Monoclonal Antibody Flow cytometry.
SEB040Hu ELISA Kit for Vimentin (VIM) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB040Hu Multiplex Assay Kit for Vimentin (VIM) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB040Hu01 ELISA Kit DIY Materials for Vimentin (VIM) Main materials for "Do It (ELISA Kit) Yourself".