ELISA Kit DIY Materials for Podocin (PDCN)

NPHS2; NPHS-2; Nephrosis 2; Idiopathic Steroid-Resistant

  • Product No.KSA938Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates, urine, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.052ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1166  US$ 1944  US$ 3499  US$ 6804  US$ 11664 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Podocin (PDCN). No significant cross-reactivity or interference between Podocin (PDCN) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Experimental and molecular pathology Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy. pubmed:29246788
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Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA938Mu01 Recombinant Podocin (PDCN) Positive Control; Immunogen; SDS-PAGE; WB.
PAA938Mu01 Polyclonal Antibody to Podocin (PDCN) WB; IHC; ICC; IP.
LAA938Mu71 Biotin-Linked Polyclonal Antibody to Podocin (PDCN) WB; IHC; ICC.
SEA938Mu ELISA Kit for Podocin (PDCN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA938Mu Multiplex Assay Kit for Podocin (PDCN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA938Mu01 ELISA Kit DIY Materials for Podocin (PDCN) Main materials for "Do It (ELISA Kit) Yourself".