ELISA Kit DIY Materials for Melatonin (MT)
- Product No.KSA908Ge11
- Organism SpeciesPan-species (General) Same name, Different species.
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma and other biological fluids.
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range12.35-1000pg/mL
- Applicable Sensitivity4.94pg/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- UOM 96T*596T*10 96T*20 96T*50 96T*100
For more details, please contact local distributors! US$ 2388
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The Abs in the kit have high sensitivity and excellent specificity for detection of Melatonin (MT). No significant cross-reactivity or interference between Melatonin (MT) and analogues was observed.
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
|Domestic Animal Endocrinology||Effects of melatonin on islet neogenesis and beta cell apoptosis in streptozotocin-induced diabetic rats: an immunohistochemical study ScienceDirect: S0739724012000112|
|General Assembly and Scientific Symposium||Biomarkers inducing changes due to microwave exposure effect on rat brain Academin: Source|
|Chinese Journal||Comparison of the flow characteristics for the honeycomb and labyrinth seal using numerical simulation Cnki: Source|
|Clinics (Sao Paulo).||The therapeutic effect of a pulsed electromagnetic field on the reproductive patterns of male Wistar rats exposed to a 2.45-GHz microwave field PubMed: 21876981|
|Journal of Radiation Research||Effects of simultaneous combined exposure to CDMA and WCDMA electromagnetic fields on serum hormone levels in rats PubMed: 23239176|
|Experimental Gerontology||Improvement of oxidative stress and immunity by melatonin: An age dependent study in golden hamster Pubmed: 23220117|
|Journal of Human Reproductive Sciences||Melatonin and its correlation with testosterone in polycystic ovarian syndrome NCBI: PMC3963309|
|Indian Journal of Experimental Biology||Daily variation in melatonin level, antioxidant activity and general immune response of peripheral blood mononuclear cells and lymphoid tissues of Indian goat Capra … Pubmed: 24851409|
|Metabolic Syndrome||Serum Melatonin Level Disturbance is Related to Metabolic Syndrome and Subclinical Arterial Dysfunction in Shift Working Healthy Men Researchgate: Source|
|Israel Journal of Veterinary Medicine||Melatonin Administration Aids Bone Healing in Diabetic Rats Ijvm: Source|
|Journal of Photochemistry and Photobiology B: Biology||Photoperiodic induced melatonin regulates immunity and expression pattern of melatonin receptor MT1 in spleen and bone marrow mononuclear cells of male golden hamster. Pubmed: 24090924|
|In Vivo.||Influence of?circadian?time?and lighting conditions on expression of melatonin?receptors?1?and?2?in?murinelymphocytes. Pubmed:25189896|
|Biomicrofluidics.||Molecularly imprinted electrochemical sensing of urinary melatonin in a microfluidic system Pubmed:25584113|
|International Journal of Advanced Research||Localization and expression of androgen receptor (AR) and melatonin membrane receptor (MT1R) in accessory sex organs of adult male golden hamster, Mesocricetus auratus: Modulation by photoperiod. Com:Source|
|Journal of Nanoparticle Research||Titanium oxide (TiO2) nanoparticles in induction of apoptosis and inflammatory response in brain Springer:Source|
|International Immunopharmacology||MT1 receptor expression and AA-NAT activity in lymphatic tissue following melatonin administration in male golden hamster Pubmed:25023124|
|Journal of Photochemistry and Photobiology B: Biology||Photoperiodic regulation of melatonin membrane receptor (MT1R) expression and steroidogenesis in testis of adult golden hamster, Mesocricetus auratus Pubmed:25255424|
|Andrologia||Melatonin prevents dexamethasone-induced testicular oxidative stress and germ cell apoptosis in golden hamster, Mesocricetus auratus Pubmed:25277062|
|Biological Rhythm Research||Reproductive seasonality in the Indian pygmy field mouse, Mus terricolor Tandfonline:Source|
|Acta Histochem||Developmental changes of melatonin receptor expression in the spleen of the chicken, Gallus domesticus PubMed: 26024931|
|Acta Histochemica||Melatonin modulates monochromatic light-induced GHRH expression in the hypothalamus and GH secretion in chicks Pubmed:26948666|
|Veterinary Word||Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers Pubmed:27051212|
|Journal of Photochemistry and Photobiology B: Biology||Effect of melatonin on monochromatic light-induced T-lymphocyte proliferation in the thymus of chickens Pubmed:27203566|
|Asian-Australas J Anim Sci.||Effect of Monochromic Light-emitting Diode Light with Different Color on the Growth and ReproductivePerformances of Breeder Geese. Pubmed:26954165|
|Revista Brasileira de Saúde e Produção Animal||Desempenho e comportamento de suínos em fase de terminação submetidos a diferentes programas de luz S1519-99402016000200272|
|journal of neuroendocrinology||Green light inhibits GnRH-I expression by stimulating the melatonin-GnIH pathway in the chick brain. pubmed:28295740|
|Revista Brasileira de Medicina do Esporte||EFFECTS OF DIURNAL AND NOCTURNAL STRENUOUS EXERCISE ON SERUM MELATONIN LEVELS S1517-86922016000600436|
|Journal of Photochemistry and Photobiology B||Role of monochromatic light on daily variation of clock gene expression in the pineal gland ofchick. pubmed:27643985|
|Journal of Oral Science||Antioxidant effects of melatonin in heart tissue after induction of experimental periodontitis inrats. pubmed:28367898|
|畜牧兽医学报||单色绿光对鸡胚褪黑激素合成的影响 DOI: 10.11843/j.issn.0366-6964.2017.05.019|
|Oxidative Medicine and Cellular Longevity||Melatonin Supplementation Lowers Oxidative Stress and Regulates Adipokines in Obese Patients on a Calorie-Restricted Diet pubmed:29142618|
|Pakistan veterinary journal||Inhibitory Effect of Melatonin on Formation of the Chicken Primordial Follicles. ISSN: 0253-8318|
|Acta histochemica||Melatonin modulates monochromatic light-induced melatonin receptor expression in the hypothalamus of chicks pubmed:28943001|
|Stem Cell Reviews and Reports||A Circadian Rhythm in both Complement Cascade (ComC) Activation and Sphingosine-1-Phosphate (S1P) Levels in Human Peripheral Blood Supports a … 10.1007:s12015-018-9836-7|
|Photochemistry and Photobiology||Effect of Monochromatic Light on Circadian Rhythm of Clock Genes in Chick Pinealocytes Pubmed:29896808|
|Reproduction In Domestic Animals||Expression of melatonin and its related synthase and membrane receptors in the oestrous corpus luteum and corpus luteum verum of sheep Pubmed:29943511|
|OCCUPATIONAL HYGIENE–PERFORMANCE COFACTOR IN POWER ENGINEERING. BIOCHEMICAL RESPONSE TO EXPOSURE TO EXTREMELY LOW … id:PLUMEE201601V06S01A0004|
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|KSA908Ge11||ELISA Kit DIY Materials for Melatonin (MT)||Main materials for "Do It (ELISA Kit) Yourself".|