ELISA Kit DIY Materials for Melatonin (MT)



The Abs in the kit have high sensitivity and excellent specificity for detection of Melatonin (MT). No significant cross-reactivity or interference between Melatonin (MT) and analogues was observed.


1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.


Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Domestic Animal Endocrinology Effects of melatonin on islet neogenesis and beta cell apoptosis in streptozotocin-induced diabetic rats: an immunohistochemical study ScienceDirect: S0739724012000112
General Assembly and Scientific Symposium Biomarkers inducing changes due to microwave exposure effect on rat brain Academin: Source
Chinese Journal Comparison of the flow characteristics for the honeycomb and labyrinth seal using numerical simulation Cnki: Source
Clinics (Sao Paulo). The therapeutic effect of a pulsed electromagnetic field on the reproductive patterns of male Wistar rats exposed to a 2.45-GHz microwave field PubMed: 21876981
Journal of Radiation Research Effects of simultaneous combined exposure to CDMA and WCDMA electromagnetic fields on serum hormone levels in rats PubMed: 23239176
Experimental Gerontology Improvement of oxidative stress and immunity by melatonin: An age dependent study in golden hamster Pubmed: 23220117
Journal of Human Reproductive Sciences Melatonin and its correlation with testosterone in polycystic ovarian syndrome NCBI: PMC3963309
Indian Journal of Experimental Biology Daily variation in melatonin level, antioxidant activity and general immune response of peripheral blood mononuclear cells and lymphoid tissues of Indian goat Capra … Pubmed: 24851409
Metabolic Syndrome Serum Melatonin Level Disturbance is Related to Metabolic Syndrome and Subclinical Arterial Dysfunction in Shift Working Healthy Men Researchgate: Source
Israel Journal of Veterinary Medicine Melatonin Administration Aids Bone Healing in Diabetic Rats Ijvm: Source
Journal of Photochemistry and Photobiology B: Biology Photoperiodic induced melatonin regulates immunity and expression pattern of melatonin receptor MT1 in spleen and bone marrow mononuclear cells of male golden hamster. Pubmed: 24090924
In Vivo. Influence of?circadian?time?and lighting conditions on expression of melatonin?receptors?1?and?2?in?murinelymphocytes. Pubmed:25189896
Biomicrofluidics. Molecularly imprinted electrochemical sensing of urinary melatonin in a microfluidic system Pubmed:25584113
International Journal of Advanced Research Localization and expression of androgen receptor (AR) and melatonin membrane receptor (MT1R) in accessory sex organs of adult male golden hamster, Mesocricetus auratus: Modulation by photoperiod. Com:Source
Journal of Nanoparticle Research Titanium oxide (TiO2) nanoparticles in induction of apoptosis and inflammatory response in brain Springer:Source
International Immunopharmacology MT1 receptor expression and AA-NAT activity in lymphatic tissue following melatonin administration in male golden hamster Pubmed:25023124
Journal of Photochemistry and Photobiology B: Biology Photoperiodic regulation of melatonin membrane receptor (MT1R) expression and steroidogenesis in testis of adult golden hamster, Mesocricetus auratus Pubmed:25255424
Andrologia Melatonin prevents dexamethasone-induced testicular oxidative stress and germ cell apoptosis in golden hamster, Mesocricetus auratus Pubmed:25277062
Biological Rhythm Research Reproductive seasonality in the Indian pygmy field mouse, Mus terricolor Tandfonline:Source
Acta Histochem Developmental changes of melatonin receptor expression in the spleen of the chicken, Gallus domesticus PubMed: 26024931
Acta Histochemica Melatonin modulates monochromatic light-induced GHRH expression in the hypothalamus and GH secretion in chicks Pubmed:26948666
Veterinary Word Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers Pubmed:27051212
Journal of Photochemistry and Photobiology B: Biology Effect of melatonin on monochromatic light-induced T-lymphocyte proliferation in the thymus of chickens Pubmed:27203566
Asian-Australas J Anim Sci. Effect of Monochromic Light-emitting Diode Light with Different Color on the Growth and ReproductivePerformances of Breeder Geese. Pubmed:26954165
Revista Brasileira de Saúde e Produção Animal Desempenho e comportamento de suínos em fase de terminação submetidos a diferentes programas de luz S1519-99402016000200272
journal of neuroendocrinology Green light inhibits GnRH-I expression by stimulating the melatonin-GnIH pathway in the chick brain. pubmed:28295740
Journal of Photochemistry and Photobiology B Role of monochromatic light on daily variation of clock gene expression in the pineal gland ofchick. pubmed:27643985
Journal of Oral Science Antioxidant effects of melatonin in heart tissue after induction of experimental periodontitis inrats. pubmed:28367898
畜牧兽医学报 单色绿光对鸡胚褪黑激素合成的影响 DOI: 10.11843/j.issn.0366-6964.2017.05.019
Oxidative Medicine and Cellular Longevity Melatonin Supplementation Lowers Oxidative Stress and Regulates Adipokines in Obese Patients on a Calorie-Restricted Diet pubmed:29142618
Pakistan veterinary journal Inhibitory Effect of Melatonin on Formation of the Chicken Primordial Follicles. ISSN: 0253-8318
Acta histochemica Melatonin modulates monochromatic light-induced melatonin receptor expression in the hypothalamus of chicks pubmed:28943001
Stem Cell Reviews and Reports A Circadian Rhythm in both Complement Cascade (ComC) Activation and Sphingosine-1-Phosphate (S1P) Levels in Human Peripheral Blood Supports a … 10.1007:s12015-018-9836-7
Photochemistry and Photobiology Effect of Monochromatic Light on Circadian Rhythm of Clock Genes in Chick Pinealocytes Pubmed:29896808
Reproduction In Domestic Animals Expression of melatonin and its related synthase and membrane receptors in the oestrous corpus luteum and corpus luteum verum of sheep Pubmed:29943511
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KSA908Ge11 ELISA Kit DIY Materials for Melatonin (MT) Main materials for "Do It (ELISA Kit) Yourself".