ELISA Kit for Melatonin (MT)
- Product No.CEA908Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range12.35-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 4.63pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 796
For more details, please contact local distributors! US$ 3580
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For more details, please contact local distributors! US$ 55692
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Melatonin (MT).
No significant cross-reactivity or interference between Melatonin (MT) and analogues was observed.
Matrices listed below were spiked with certain level of Melatonin (MT) and the recovery rates were calculated by comparing the measured value to the expected amount of Melatonin (MT) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Melatonin (MT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Melatonin (MT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Melatonin (MT) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Domestic Animal Endocrinology||Effects of melatonin on islet neogenesis and beta cell apoptosis in streptozotocin-induced diabetic rats: an immunohistochemical study ScienceDirect: S0739724012000112|
|General Assembly and Scientific Symposium||Biomarkers inducing changes due to microwave exposure effect on rat brain Academin: Source|
|Chinese Journal||Comparison of the flow characteristics for the honeycomb and labyrinth seal using numerical simulation Cnki: Source|
|Clinics (Sao Paulo).||The therapeutic effect of a pulsed electromagnetic field on the reproductive patterns of male Wistar rats exposed to a 2.45-GHz microwave field PubMed: 21876981|
|Journal of Radiation Research||Effects of simultaneous combined exposure to CDMA and WCDMA electromagnetic fields on serum hormone levels in rats PubMed: 23239176|
|Experimental Gerontology||Improvement of oxidative stress and immunity by melatonin: An age dependent study in golden hamster Pubmed: 23220117|
|Journal of Human Reproductive Sciences||Melatonin and its correlation with testosterone in polycystic ovarian syndrome NCBI: PMC3963309|
|Indian Journal of Experimental Biology||Daily variation in melatonin level, antioxidant activity and general immune response of peripheral blood mononuclear cells and lymphoid tissues of Indian goat Capra … Pubmed: 24851409|
|Metabolic Syndrome||Serum Melatonin Level Disturbance is Related to Metabolic Syndrome and Subclinical Arterial Dysfunction in Shift Working Healthy Men Researchgate: Source|
|Israel Journal of Veterinary Medicine||Melatonin Administration Aids Bone Healing in Diabetic Rats Ijvm: Source|
|Journal of Photochemistry and Photobiology B: Biology||Photoperiodic induced melatonin regulates immunity and expression pattern of melatonin receptor MT1 in spleen and bone marrow mononuclear cells of male golden hamster. Pubmed: 24090924|
|In Vivo.||Influence of?circadian?time?and lighting conditions on expression of melatonin?receptors?1?and?2?in?murinelymphocytes. Pubmed:25189896|
|Biomicrofluidics.||Molecularly imprinted electrochemical sensing of urinary melatonin in a microfluidic system Pubmed:25584113|
|International Journal of Advanced Research||Localization and expression of androgen receptor (AR) and melatonin membrane receptor (MT1R) in accessory sex organs of adult male golden hamster, Mesocricetus auratus: Modulation by photoperiod. Com:Source|
|Journal of Nanoparticle Research||Titanium oxide (TiO2) nanoparticles in induction of apoptosis and inflammatory response in brain Springer:Source|
|International Immunopharmacology||MT1 receptor expression and AA-NAT activity in lymphatic tissue following melatonin administration in male golden hamster Pubmed:25023124|
|Journal of Photochemistry and Photobiology B: Biology||Photoperiodic regulation of melatonin membrane receptor (MT1R) expression and steroidogenesis in testis of adult golden hamster, Mesocricetus auratus Pubmed:25255424|
|Andrologia||Melatonin prevents dexamethasone-induced testicular oxidative stress and germ cell apoptosis in golden hamster, Mesocricetus auratus Pubmed:25277062|
|Biological Rhythm Research||Reproductive seasonality in the Indian pygmy field mouse, Mus terricolor Tandfonline:Source|
|Acta Histochem||Developmental changes of melatonin receptor expression in the spleen of the chicken, Gallus domesticus PubMed: 26024931|
|Acta Histochemica||Melatonin modulates monochromatic light-induced GHRH expression in the hypothalamus and GH secretion in chicks Pubmed:26948666|
|Veterinary Word||Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers Pubmed:27051212|
|Journal of Photochemistry and Photobiology B: Biology||Effect of melatonin on monochromatic light-induced T-lymphocyte proliferation in the thymus of chickens Pubmed:27203566|
|Asian-Australas J Anim Sci.||Effect of Monochromic Light-emitting Diode Light with Different Color on the Growth and ReproductivePerformances of Breeder Geese. Pubmed:26954165|
|Revista Brasileira de Saúde e Produção Animal||Desempenho e comportamento de suínos em fase de terminação submetidos a diferentes programas de luz S1519-99402016000200272|
|journal of neuroendocrinology||Green light inhibits GnRH-I expression by stimulating the melatonin-GnIH pathway in the chick brain. pubmed:28295740|
|Revista Brasileira de Medicina do Esporte||EFFECTS OF DIURNAL AND NOCTURNAL STRENUOUS EXERCISE ON SERUM MELATONIN LEVELS S1517-86922016000600436|
|Journal of Photochemistry and Photobiology B||Role of monochromatic light on daily variation of clock gene expression in the pineal gland ofchick. pubmed:27643985|
|Journal of Oral Science||Antioxidant effects of melatonin in heart tissue after induction of experimental periodontitis inrats. pubmed:28367898|
|畜牧兽医学报||单色绿光对鸡胚褪黑激素合成的影响 DOI: 10.11843/j.issn.0366-6964.2017.05.019|
|Oxidative Medicine and Cellular Longevity||Melatonin Supplementation Lowers Oxidative Stress and Regulates Adipokines in Obese Patients on a Calorie-Restricted Diet pubmed:29142618|
|Pakistan veterinary journal||Inhibitory Effect of Melatonin on Formation of the Chicken Primordial Follicles. ISSN: 0253-8318|
|Acta histochemica||Melatonin modulates monochromatic light-induced melatonin receptor expression in the hypothalamus of chicks pubmed:28943001|
|Stem Cell Reviews and Reports||A Circadian Rhythm in both Complement Cascade (ComC) Activation and Sphingosine-1-Phosphate (S1P) Levels in Human Peripheral Blood Supports a … 10.1007:s12015-018-9836-7|
|Photochemistry and Photobiology||Effect of Monochromatic Light on Circadian Rhythm of Clock Genes in Chick Pinealocytes Pubmed:29896808|
|Reproduction In Domestic Animals||Expression of melatonin and its related synthase and membrane receptors in the oestrous corpus luteum and corpus luteum verum of sheep Pubmed:29943511|
|OCCUPATIONAL HYGIENE–PERFORMANCE COFACTOR IN POWER ENGINEERING. BIOCHEMICAL RESPONSE TO EXPOSURE TO EXTREMELY LOW … id:PLUMEE201601V06S01A0004|
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