ELISA Kit DIY Materials for D-Dimer (D2D)

D 2 Dimer


The Abs in the kit have high sensitivity and excellent specificity for detection of D-Dimer (D2D). No significant cross-reactivity or interference between D-Dimer (D2D) and analogues was observed.


1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.


Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
22 Determinants of the thrombogenic potential of multiwalled carbon nanotubes ScienceDirect: S0142961211004819
Journal of Thrombosis and Haemostasis Exogenous Activated Protein C Inhibits the Progression of Diabetic Nephropathy Pubmed: 22236035
PLOS ONE Protective Effect of Curcumin on Pulmonary and Cardiovascular Effects Induced by Repeated Exposure to Diesel Exhaust Particles in Mice PlosOne: Source
Haemetology Clinical impact of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations among sickle cell disease patients of Central India Pubmed: 23992124
Journal of Trauma and Acute Care Surgery Assessment of coagulopathy, endothelial injury, and inflammation after traumatic brain injury and hemorrhage in a porcine model Lww:Source
Journal of Thrombosis and Haemostasis Protein S exacerbates alcoholic hepatitis by stimulating liver natural killer T cells Pubmed:25399514
Inflammation.? Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis Pubmed:25448261
Medical Science Monitor Impact of β-Adrenoceptor Blockade on Systemic Inflammation and Coagulation Disturbances in Rats with Acute Traumatic Coagulopathy Pubmed:25676919
Blood Intraperitoneal administration of activated protein C prevents postsurgical adhesion band formation. PubMed: 25575539
Am J Physiol Regul Integr Comp Physiol Procoagulant and Fibrinolytic Activity after Polytrauma in Rat PubMed: 26632604
hypertension Research Upregulation of canonical transient receptor potential channel in the pulmonary arterial smooth muscle of a chronic thromboembolic pulmonary hypertension rat model PubMed: 26155749
Molecular Nutrition & Food Research Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors PubMed: 25546502
JCI Insight. KLF2 and KLF4 control endothelial identity and vascular integrity. pubmed:28239661
The American Society of Anesthesiologists Activated protein C drives the hyperfibrinolysis of acute traumatic coagulopathy pubmed:27841821
32 Activated TAFI Promotes the Development of Chronic Thromboembolic Pulmonary Hypertension: A Possible Novel Therapeutic Target pubmed:28289017
The American Journal of Pathology Inhibition of Cell Apoptosis and Amelioration of Pulmonary Fibrosis by Thrombomodulin pubmed:28739343
Atherosclerosis Low dose of alcohol attenuates pro-atherosclerotic activity of thrombin. pubmed:28923781
28 Lactadherin Promotes Microvesicle Clearance to Prevent Coagulopathy and Improves Survival of Severe TBI Mice pubmed:29162596
Journal of Thrombosis and Thrombolysis Guided longer pulses from a diagnostic ultrasound and intraclot microbubble enhanced catheter-directed thrombolysis in vivo. pubmed:28417266
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