Wide-range ELISA Kit for D-Dimer (D2D)
D 2 Dimer
- Product No.WEA506Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample Typeplasma
- Test MethodCompetitive Inhibition
- Assay Length3h
- Detection Range12.35-1,000ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.53ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 547
For more details, please contact local distributors! US$ 2462
For more details, please contact local distributors! US$ 4650
For more details, please contact local distributors! US$ 38290
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Wide-range D-Dimer (D2D).
No significant cross-reactivity or interference between Wide-range D-Dimer (D2D) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Wide-range D-Dimer (D2D) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range D-Dimer (D2D) in samples.
|Matrix||Recovery range (%)||Average(%)|
|sodium citrate plasma(n=5)||93-101||98|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range D-Dimer (D2D) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range D-Dimer (D2D) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range D-Dimer (D2D) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|sodium citrate plasma(n=5)||83-94%||94-102%||95-103%||84-103%|
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|22||Determinants of the thrombogenic potential of multiwalled carbon nanotubes ScienceDirect: S0142961211004819|
|Journal of Thrombosis and Haemostasis||Exogenous Activated Protein C Inhibits the Progression of Diabetic Nephropathy Pubmed: 22236035|
|PLOS ONE||Protective Effect of Curcumin on Pulmonary and Cardiovascular Effects Induced by Repeated Exposure to Diesel Exhaust Particles in Mice PlosOne: Source|
|Haemetology||Clinical impact of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations among sickle cell disease patients of Central India Pubmed: 23992124|
|Journal of Trauma and Acute Care Surgery||Assessment of coagulopathy, endothelial injury, and inflammation after traumatic brain injury and hemorrhage in a porcine model Lww:Source|
|Journal of Thrombosis and Haemostasis||Protein S exacerbates alcoholic hepatitis by stimulating liver natural killer T cells Pubmed:25399514|
|Inflammation.?||Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis Pubmed:25448261|
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|Blood||Intraperitoneal administration of activated protein C prevents postsurgical adhesion band formation. PubMed: 25575539|
|Am J Physiol Regul Integr Comp Physiol||Procoagulant and Fibrinolytic Activity after Polytrauma in Rat PubMed: 26632604|
|hypertension Research||Upregulation of canonical transient receptor potential channel in the pulmonary arterial smooth muscle of a chronic thromboembolic pulmonary hypertension rat model PubMed: 26155749|
|Molecular Nutrition & Food Research||Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors PubMed: 25546502|
|JCI Insight.||KLF2 and KLF4 control endothelial identity and vascular integrity. pubmed:28239661|
|The American Society of Anesthesiologists||Activated protein C drives the hyperfibrinolysis of acute traumatic coagulopathy pubmed:27841821|
|32||Activated TAFI Promotes the Development of Chronic Thromboembolic Pulmonary Hypertension: A Possible Novel Therapeutic Target pubmed:28289017|
|The American Journal of Pathology||Inhibition of Cell Apoptosis and Amelioration of Pulmonary Fibrosis by Thrombomodulin pubmed:28739343|
|Atherosclerosis||Low dose of alcohol attenuates pro-atherosclerotic activity of thrombin. pubmed:28923781|
|28||Lactadherin Promotes Microvesicle Clearance to Prevent Coagulopathy and Improves Survival of Severe TBI Mice pubmed:29162596|
|Journal of Thrombosis and Thrombolysis||Guided longer pulses from a diagnostic ultrasound and intraclot microbubble enhanced catheter-directed thrombolysis in vivo. pubmed:28417266|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
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