ELISA Kit DIY Materials for Triiodothyronine (T3)

3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin


The Abs in the kit have high sensitivity and excellent specificity for detection of Triiodothyronine (T3). No significant cross-reactivity or interference between Triiodothyronine (T3) and analogues was observed.


1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.


Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support Pack



Magazine Citations
Aquatic Toxicology Bioconcentration and metabolism of decabromodiphenyl ether (BDE-209) result in thyroid endocrine disruption in zebrafish larvae ScienceDirect: S0166445X12000215
Aquatic Toxicology Exposure of zebrafish embryos/larvae to TDCPP alters concentrations of thyroid hormones and transcriptions of genes involved in the hypothalamic–pituitary–thyroid axis PubMed: 23220413
European Journal of Zoological Research Effect of dietary tryptophan on plasma growth hormone and thyroid hormone in broiler chicks Scholarsresearchlibrary: Source
Life Science Journal Can Nigella Sativa oil (NSO) reverse hypothyroid status induced by PTU in rat? biochemical and histological studies Lifesciencesite: Source
PLoS One A Comparative Genotoxicity Study of a Supraphysiological Dose of Triiodothyronine (T3) in Obese Rats Subjected to Either Calorie-Restricted Diet or Hyperthyroidism Pubmed: 23468891
Aquat Toxicol Thyroid endocrine system disruption by pentachlorophenol: an in vitro and in vivo assay. ScienceDirect: S0166445X13002129
Aquatic Toxicology Impact of co-exposure with lead and decabromodiphenyl ether (BDE-209) on thyroid function in zebrafish larvae Pubmed:25456233
Chemosphere Exposure to2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) alters thyroid hormone levels and thyroid hormone-reCavia (Guinea pig )lated gene transcription in manila clam Ruditapes philippinarum science:S0045653516302016
Toxicological Sciences Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring pubmed:27803384
Sci Rep Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. pubmed:28322267
Aquat Toxicol. Developmental toxicity and thyroid hormone-disrupting effects of 2,4-dichloro-6-nitrophenol in Chinese rare minnow (Gobiocypris rarus). pubmed:28187359
Bulletin of the World Health Organization Endemic goitre in the Sudan despite longstanding programmes for the control of iodine deficiency disorders handle:123456789
Biological Rhythm Research Age-related and seasonal variations in plasma uncarboxylated osteocalcin in male Murrah buffaloes 09291016.2016.1275393
Toxicological Sciences Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reduction pubmed:28973666
Environmental Pollution Transgenerational endocrine disruption and neurotoxicity in zebrafish larvae after parental exposure to binary mixtures of decabromodiphenyl ether (BDE-209) and lead 10.1016/j.envpol.2017.06.053
Sciencepaper online 四溴双酚A对菲律宾蛤仔的生长抑制效应机理Growth inhibition Effects of TBBPA on juvenile manila clam Ruditapes philippinarum 5790267088906950000
Tropical Journal of Pharmaceutical Research Neuroprotective effects of thymoquinone against cerebellar histopathological changes in propylthiouracil-induced hypothyroidism in adult rats 10.4314/tjpr.v16i5.9
s-space Screening thyroid hormone disrupting effects of benzophenones using GH3, FRTL-5 cells and zebrafish 10371/137708
s-space Thyroid disrupting effects and associated mechanisms of TCPP in GH3 cell line and zebrafish (Danio rerio) larva and adult 10371/137708
Environmental Pollution Time-dependent inhibitory effects of Tris(1, 3-dichloro-2-propyl) phosphate on growth and transcription of genes involved in the GH/IGF axis, but not the HPT axis, in female zebrafish. pubmed:28624628
Environmental Science & Technology Thyroid hormone-disrupting potentials of major benzophenones in two cell lines (GH3 and FRTL-5) and embryo-larval zebrafish Pubmed:29995391
Environmental Pollution Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population Pubmed:29525625
Toxicological Sciences Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons 10.1093:toxsci
Frontiers in Molecular Neuroscience Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice Pubmed:29403355
Drug metabolism and disposition Activation of 5-HT1A receptors in the hypothalamic paraventricular nuclei (PVN) negatively regulates cytochrome P450 expression and activity in rat liver Pubmed:29555828
Archives Animal Breeding Age-related changes in testicular parameters and their relationship to thyroid hormones and testosterone in male Murrah buffaloes 10.5194:aab-61-191-2018
Indian Journal of Animal Sciences Endurance exercise causes adverse changes in some hematological and physio-biochemical indices in ponies under high altitude stress condition :
Environmental Pollution Transgenerational thyroid endocrine disruption induced by bisphenol S affects the early development of zebrafish offspring Pubmed: 30243188
Cell Reports Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice Pubmed: 30404008
Drug Metabolism and Disposition Serotonin receptors of 5-HT2 type in the hypothalamic arcuate nuclei (ARC) positively regulate liver cytochrome P450 via stimulation of the growth hormone-releasing … Pubmed: 30518657
Science of the Total Environment Parental exposure to 6: 2 chlorinated polyfluorinated ether sulfonate (F53B) induced transgenerational thyroid hormone disruption in zebrafish Doi: 10.1016/j.scitotenv.2019.02.198
Theriogenology  Thyroid hormone profiles and TSH evaluation during early pregnancy and the transition period in dairy cows Pubmed: 30784791
Chemosphere Inhibition of growth in juvenile manila clam Ruditapes philippinarum: Potential adverse outcome pathway of TBBPA Pubmed: 30844590
Aquatic Toxicology Effects of cis-bifenthrin enantiomers on the growth, behavioral, biomarkers of oxidative damage and bioaccumulation in Xenopus laevis Pubmed: 31276910
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