ELISA Kit DIY Materials for Beta-2-Microglobulin (b2M)

BMG; B2MG

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Beta-2-Microglobulin (b2M). No significant cross-reactivity or interference between Beta-2-Microglobulin (b2M) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
BioMed Research International The Renal Effects of Vanadate Exposure: Potential Biomarkers and Oxidative Stress as a Mechanism of Functional Renal Disorders—Preliminary Studies Hindawi: 740105
BMC Nephrology Absence of chloride intracellular channel 4 (CLIC4) predisposes to acute kidney injury but has minimal impact on recovery Biomedcentral: Source
Nature Communications Mef2C restrains microglial inflammatory response and is lost in brain ageing in an IFN-I-dependent manner. pubmed:28959042
PLoS One Urine proteome analysis by C18 plate–matrix-assisted laser desorption/ionization time-of-flight mass spectrometry allows noninvasive differential diagnosis … Pubmed:30024955
Acta Anaesthesiologica Scandinavica Early prognostic factors in septic shock cancer patients: a prospective study with a proteomic approach Pubmed:29315472
Toxicology Mechanisms and Methods Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar rats Pubmed: 30426860
Journal of Clinical Medicine Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese Patients Pubmed: 30486327
Journal of Proteomics iTRAQ-based analysis for the identification of MARCH8 targets in human esophageal squamous cell carcinoma 33540066
Antioxidants Diminishment of Nrf2 Antioxidative Defense Aggravates Nephrotoxicity of Melamine and Oxalate Coexposure. Antioxidants 2021, 10, 1464 34573096
Int Arch Occup Environ Health Biomarkers of cadmium exposure and renal function in estuarine adult villagers 34773507
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