ELISA Kit DIY Materials for Matrix Metalloproteinase 3 (MMP3)

SL1; STMY; STMY1; STR1; Progelatinase; Stromelysin 1; Transin-1

  • Product No.KSA101Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range31.2-2000pg/mL
  • Applicable Sensitivity13.1pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 806  US$ 1344  US$ 2419  US$ 4704  US$ 8064 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 3 (MMP3). No significant cross-reactivity or interference between Matrix Metalloproteinase 3 (MMP3) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Journal of Bone and Joint Surgery Experimental osteoarthritis induced by surgical realignment of the patella in BALB/c mice PubMed: 21119180
Infection and Immunity Bovine Respiratory Syncytial Virus and Histophilus somni Interaction at the Alveolar Barrier Pubmed: 23649093
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source
China Pharmacy Influence of Tongfengtai Granules on Inflammatory Factor in Acute Gouty Arthritis Model Rats Source
Thromb Haemost. Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke Pubmed:24671655
Connective Tissue Research Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes Pubmed:25333747
physiological research The skin remodeling in type 1 diabetes and insulin resistance animal models PubMed: 26047379
Physiol Res The skin remodeling in type 1 diabetes and insulin resistance animal models. PubMed: 26047379
Cellular & Molecular Immunology Effect of ebosin on modulating interleukin-2&bgr;-induced inflammatory responses in rat fibroblast-like synoviocytes PubMed: 25938977
Biol Res The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis PubMed: 26578310
J Orthop Res. Tetrandrine suppresses articular inflammatory response by inhibiting pro-inflammatory factors via NF-κB inactivation. Pubmed:26748661
Cellular & Molecular Immunology Effect of ebosin on modulating interleukin-1β-induced inflammatory responses in rat fibroblast-like synoviocytes pubmed:25938977
Journal of Orthopaedic Research Tetrandrine suppresses articular inflammatory response by inhibiting pro‐inflammatory factors via NF‐κB inactivation pubmed:26748661
Mediators of Inflammation Interleukin-29 Enhances Synovial Inflammation and Cartilage Degradation in Osteoarthritis. pubmed:27433031
Molecular Medicine Reports Icariin inhibits MMP‑1, MMP‑3 and MMP‑13 expression through MAPK pathways in IL‑1β‑stimulated SW1353 chondrosarcoma cells pubmed:28447732
der Christian-Albrechts-Universität zu Kiel Wirkung schonender Laserverfahren auf den Komplexaus retinalem Pigmentepithel, Bruch´scher Membranund Choroidea - mögliche Therapieoption der frühen altersabhängigen Makuladegeneration 1133074014:34
REDVET - Revista electrónica de Veterinaria Measurement of Metalloproteinase-3 levels in synovial fluid from horses as biomarker of joint disease E-ISSN: 1695-7504
REDVET - Revista electrónica de Veterinaria Measurement of Metalloproteinase-3 levels in synovial fluid from horses as biomarker of joint disease - Medición de los niveles de Metaloproteinasa-3 en liquido sinovial comobiomarcador de enfermedad articular ISSN 1695-7504
Molecular Pharmacology Dexamethasone Down-regulates Endothelin Receptors and Reduces Endothelin-induced Production of Matrix Metalloproteinases in Cultured Rat Astrocytes 10.1124/mol.116.107300
Theranostics Targeting intracellular MMPs efficiently inhibits tumor metastasis and angiogenesis Pubmed:29774078
Chinese Journal of Integrative Medicine Electroacupuncture Delays Cartilage Degeneration by Modulating Nuclear Factor-κB Signaling Pathway Pubmed: 30656598
Effects of a hyperimmunized egg product on voluntary physical activity levels, serum inflammatory markers, and owner perception of joint pain of dogs with?…
PLoS One Co-culture of osteochondral explants and synovial membrane as in vitro model for osteoarthritis Pubmed: 30939166
ACS Biomaterials Science & Engineering Fluorogenic Probe for Detecting Active Matrix Metalloproteinase-3 (MMP-3) in Plasma and Peripheral Blood Neutrophils to Indicate the Severity of Rheumatoid …
World Journal of Pediatrics Regulation of JAK/STAT signal pathway by miR-21 in the pathogenesis of juvenile idiopathic arthritis Pubmed: 31641939
International Journal of Molecular Sciences Matrix Metalloproteinase-3 is Key Effector of TNF-α-Induced Collagen Degradation in Skin Pubmed: 31652545
JOURNAL OF CELLULAR BIOCHEMISTRY Teneligliptin inhibits IL‐1β‐induced degradation of extracellular matrix in human chondrocytes Pubmed: 32162384
Bone Research Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis Pubmed: 32047705
Scientific Reports Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis Pubmed: 31980722
Alexandria Journal for Veterinary Sciences The Clinicopathological Picture of Some Postpartum Disorders in Barki Ewes.
Identification of Polyphenolic Compounds and Hepatoprotective Activity of Artichoke (Cynara Scolymus L.) Edible Part Extracts in Rats
Food Funct Undenatured type II collagen prevents and treats the osteoarthritis and motor function degradation in T2DM patients and db/db mice 33890588
journal biomedicine translational research Peanut (Arachis hypogaea) Shells Extract and Apis dorsata Honey Reduce Matrix Metalloproteinase-3 in Monosodium Iodoacetate-Induced Osteoarthritic?¡­
JOURNAL OF ANIMAL SCIENCE Physical Activity Patterns of Free Living Dogs Diagnosed with Osteoarthritis 34216471
Osteoarthritis Cartilage A systematic review on the potential value of synovial fluid biomarkers to predict clinical outcomes in cartilage repair treatments Pubmed:35618204
Phytomedicine Transcriptomic profiling revealed the role of apigenin-4′-O-α-L-rhamnoside in inhibiting the activation of rheumatoid arthritis fibroblast-like synoviocytes via MAPK … Pubmed:35660352
Journal of Immunology Research Ebosin Attenuates the Inflammatory Responses Induced by TNF-α through Inhibiting NF-κB and MAPK Pathways in Rat Fibroblast-Like Synoviocytes Pubmed:35340587
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KSA101Hu01 ELISA Kit DIY Materials for Matrix Metalloproteinase 3 (MMP3) Main materials for "Do It (ELISA Kit) Yourself".