ELISA Kit for Apelin (APLN)
XNPEP2; APEL; APJ endogenous ligand
- Product No.CED066Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range12.35-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 4.84pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 760
For more details, please contact local distributors! US$ 3420
For more details, please contact local distributors! US$ 6460
For more details, please contact local distributors! US$ 53200
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Apelin (APLN).
No significant cross-reactivity or interference between Apelin (APLN) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Apelin (APLN) and the recovery rates were calculated by comparing the measured value to the expected amount of Apelin (APLN) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apelin (APLN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apelin (APLN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Apelin (APLN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Clin Exp Hypertens.||Individual and Concomitant Effects of Cardioprotective Programs on Cardiac Apelinergic System and Oxidative State in └-NAME-Induced Hypertension Informahealthcare: Source|
|African Journal of Pharmacy and Pharmacology||Cardioprotective effects of aerobic regular exercise against doxorubicin-induced oxidative stress in rat. Academicjournals: Source|
|Zahedan Journal of research in Medical Sciences||Improvement of Kidney Apelin and Apelin Receptor in Nitro-L-Arginine-Methyl Ester Induced Hypertension Rats Zjrms: Source|
|Endocr J.||CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes Pubmed:25168658|
|Mol Metab||TIMP3 interplays with apelin to regulate cardiovascular metabolism in hypercholesterolemic mice PubMed: 26500845|
|Journal of medicinal food||Regulation of GIP and Ghrelin in Healthy Subjects Fed on Sun-Dried Raisins: A Pilot Study with a Crossover Trial Design. pubmed:28170279|
|Arch Physiol Biochem||Associations of apelin, leptin, irisin, ghrelin, insulin, glucose levels, and lipid parameters with physical activity during eight weeks of regular exercise training Pubmed: 31290696|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|RPD066Ra01||Recombinant Apelin (APLN)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAD066Ra01||Polyclonal Antibody to Apelin (APLN)||WB; IHC; ICC; IP.|
|LAD066Ra71||Biotin-Linked Polyclonal Antibody to Apelin (APLN)||WB; IHC; ICC.|
|MAD066Ra21||Monoclonal Antibody to Apelin (APLN)||WB; IHC; ICC; IP.|
|SED066Ra||ELISA Kit for Apelin (APLN)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|CED066Ra||ELISA Kit for Apelin (APLN)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSD066Ra11||ELISA Kit DIY Materials for Apelin (APLN)||Main materials for "Do It(ELISA Kit) Yourself"|