ELISA Kit for Glucagon (GCG)
GC; GLP1; GLP2; GRPP; Glicentin-Related Polypeptide; Glucagen; Oxyntomodulin; Incretin hormone
- Product No.CEB266Ga
- Organism SpeciesChicken (Gallus) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range18.52-1,500pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 7.46pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 722
For more details, please contact local distributors! US$ 3249
For more details, please contact local distributors! US$ 6137
For more details, please contact local distributors! US$ 50540
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Glucagon (GCG).
No significant cross-reactivity or interference between Glucagon (GCG) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Glucagon (GCG) and the recovery rates were calculated by comparing the measured value to the expected amount of Glucagon (GCG) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucagon (GCG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucagon (GCG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glucagon (GCG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Molecular Metabolism||PER2 promotes glucose storage to liver glycogen during feeding and acute fasting by inducing Gys2 PTG and GL expression PubMed: PMC3773838|
|Exp Biol Med (Maywood).||Increasing glucagon secretion could antagonize the action of exogenous insulin for glycemic control in streptozocin-induced diabetic rhesus monkeys. Pubmed: 23760004|
|European Journal of Endocrinology||Specificity and sensitivity of commercially available assays for glucagon and oxyntomodulin measurement in human Eje-online: Source|
|American Diabetes Association||IL-21 is a major negative regulator of IRF4-dependent lipolysis affecting Tregs in adipose tissue and systemic insulin sensitivity Pubmed:24430438|
|Journal of Pharmacy and Pharmacology||Enzogenol improves diabetes-related metabolic change in C57BL/KsJ-db/db?mice, a model of type 2 diabetes mellitus Pubmed:24611864|
|European Journal of Pharmacology||Subcutaneous administration of liraglutide ameliorates learning and memory impairment by modulating tau hyperphosphorylation via the glycogen synthase kinase-3尾 pathway in an amyloid 尾 protein induced alzheimer disease mouse model Pubmed:27131827|
|Appl Physiol Nutr Metab||The glucoreCavia (Guinea pig )latory response to high-intensity aerobic exercise following training in rats with insulin-treated type 1 diabetes mellitus Pubmed:27175938|
|American Journal of Translational Research||Subcutaneous liraglutide ameliorates methylglyoxal-induced Alzheimer-like tau pathology and cognitive impairment by modulating tau hyperphosphorylation and glycogen synthase kinase-3β. pubmed:28337257|
|Biomedicine & Pharmacotherapy||Vitamin D3 intake as regulator of insulin degrading enzyme and insulin receptor phosphorylationin diabetic rats. pubmed:27930980|
|Diabetologia||Ginsenoside Rg5 attenuates hepatic glucagon response via suppression of succinate-associated HIF-1α induction in HFD-fed mice pubmed:28280902|
|International Journal of Molecular Sciences||Effects of Acute Cold Stress on Liver O-GlcNAcylation and Glycometabolism in Mice Pubmed: 30231545|
|European Journal of Pharmacology||Exogenous obestatin decreases beta-cell apoptosis and alfa-cell proliferation in high fat diet and streptozotocin induced type 2 diabetic rats Pubmed: 30776368|
|Stress||Regulating glycolysis, the TLR4 signal pathway and expression of RBM3 in mouse liver in response to acute cold exposure Pubmed: 30821572|
|international journal of molecular sciences||Effects of Cold-inducible RNA-binding Protein (CIRP) on Liver Glycolysis during Acute Cold Exposure in C57BL/6 Mice Pubmed: 30909542|
|Diabetes-Metabolism Research and Reviews||Liraglutide Combined with Human Umbilical Cord Mesenchymal Stem Cell Transplantation inhibits beta‐cells apoptosis via mediating the ASK1/JNK/BAX pathway in Pubmed: 31411368|
|CELL DISCOVERY||In vitro expansion of pancreatic islet clusters facilitated by hormones and chemicals Pubmed: 32284878|
|endocrine journal||Mice with nucleus tractus solitarius injury induced by chronic restraint stress present impaired ability to raise blood glucose and glucagon levels when blood glucose … Pubmed: 32249244|
|Frontiers in Endocrinology||RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis Pubmed: 32328034|
|Molecular Medicine Reports||Acarbose ameliorates spontaneous type‑2 diabetes in db/db mice by inhibiting PDX‑1 methylation Pubmed: 33236139|
|CHEMICO-BIOLOGICAL INTERACTIONS||Modulatory role of imatinib mesylate on pancreatic β-cells' secretory functions in an STZ rat model of diabetes mellitus Pubmed: 32710900|
|Catalog No.||Related products for research use of Chicken (Gallus) Organism species||Applications (RESEARCH USE ONLY!)|
|RPB266Ga01||Recombinant Glucagon (GCG)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAB266Ga01||Polyclonal Antibody to Glucagon (GCG)||WB; IHC; ICC; IP.|
|MAB266Ga21||Monoclonal Antibody to Glucagon (GCG)||WB; IHC; ICC; IP.|
|CEB266Ga||ELISA Kit for Glucagon (GCG)||Enzyme-linked immunosorbent assay for Antigen Detection.|