ELISA Kit for Galanin (GAL)

GALN; GLNN; GMAP; Galanin Prepropeptide; Galanin-Message-Associated Peptide

Specificity

This assay has high sensitivity and excellent specificity for detection of Galanin (GAL).
No significant cross-reactivity or interference between Galanin (GAL) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Galanin (GAL) and the recovery rates were calculated by comparing the measured value to the expected amount of Galanin (GAL) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-94 91
EDTA plasma(n=5) 81-94 86
heparin plasma(n=5) 95-102 99

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galanin (GAL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galanin (GAL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Galanin (GAL) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 84-98% 95-105% 99-105% 84-95%
EDTA plasma(n=5) 80-105% 84-104% 93-101% 98-105%
heparin plasma(n=5) 92-104% 84-92% 80-101% 87-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

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Magazine Citations
Progress in Neuro-Psychopharmacology and Biological Psychiatry Decreased galanin serum levels are associated with alcohol-craving during withdrawal ScienceDirect: S0278584610005191
Clinical Biochemistry Circulating galanin levels are increased in patients with gestational diabetes mellitus PubMed: 23266294
Peptides Endogenous galanin as a novel biomarker to predict gestational diabetes mellitus. Pubmed: 24503374
Gynecological Endocrinology Circulating galanin and IL-6 concentrations in gestational diabetes mellitus Pubmed:24397394
Eat Behav Association between circulating levels of galanin and pre-pregnancy body mass index in patients with gestational diabetes mellitus PubMed: 26172564
Cell Biochemistry and Biophysics The Effects of Levetiracetam on Cerebrospinal Fluid and Plasma NPY and GAL, and on the Components of Stress Response System, hs-CRP, and S100B Protein in Serum of Patients with Refractory Epilepsy Article: 10.1007
Journal of Addiction & Prevention Low Galanin Serum Levels are Associated with Anxiety in Opioid-Maintained Patients 688687ca8d5a1d03ed060f761328001ca673.pdf
Oncology Letter Colorectal cancer patients exhibit increased levels of galanin in serum and colon tissues pubmed:27899999
Scientific Reports Serum Galanin Levels in Young Healthy Lean and Obese Non-Diabetic Men during an OralGlucose Tolerance Test. pubmed:27550417
Journal of Clinical Investigation  Adrenomedullin and Galanin Responses to Orthostasis in Older Persons pubmed:28796366
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