ELISA Kit for Calcitonin Gene Related Peptide (CGRP)
CALCB; CGRP2; CALC2; CGRP-II; Calcitonin Gene Related Peptide 2; Calcitonin 2; Calcitonin Related Polypeptide Beta
- Product No.CEA876Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range12.35-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 4.65pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 684
For more details, please contact local distributors! US$ 3078
For more details, please contact local distributors! US$ 5814
For more details, please contact local distributors! US$ 47880
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Calcitonin Gene Related Peptide (CGRP).
No significant cross-reactivity or interference between Calcitonin Gene Related Peptide (CGRP) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Calcitonin Gene Related Peptide (CGRP) and the recovery rates were calculated by comparing the measured value to the expected amount of Calcitonin Gene Related Peptide (CGRP) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcitonin Gene Related Peptide (CGRP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcitonin Gene Related Peptide (CGRP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Calcitonin Gene Related Peptide (CGRP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Evidence-Based Complementary and Alternative Medicine||Alstonia scholaris R. Br. Significantly Inhibits Retinoid-Induced Skin Irritation In Vitro and In Vivo. Hindawi: 190370|
|The Journal of Headache and Pain||Increased levels of CGRP in peripheral blood in women with chronic migraine: A reliable biological marker PubMed: PMC3620076|
|Headache: The Journal of Head and Face Pain||CGRP and VIP Levels as Predictors of Efficacy of Onabotulinumtoxin Type A in Chronic Migraine Onlinelibrary: head.12372|
|PLoS One.||Ultraviolet B Irradiation Reduces the Expression of Adiponectin in Ovarial Adipose Tissues through Endocrine Actions of Calcitonin Gene-Related Peptide-Induced Serum Amyloid A Pubmed:Pmc4028234|
|Int J Vasc Med.||Prognostic significance of circulating and endothelial progenitor cell markers in type 2 diabetic foot. Pubmed:24624298|
|Arch Gynecol Obstet.||Calcitonin gene-and parathyroid hormone-related peptides in normotensive and preeclamptic pregnancies: a nested case–control study Pubmed:24943060|
|Pain||OnabotulinumtoxinA decreases interictal CGRP plasma levels in patients with chronic migraine PubMed: 25735000|
|Molecular Medicine Reports||Exogenous α-calcitonin gene-related peptide attenuates lipopolysaccharide-induced acute lung injury in rats PubMed: 25892548|
|Annals of Allergy, Asthma and Immunology||Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitis Pubmed:27156748|
|Cephalalgia.||Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patients Pubmed:27250233|
|Cardiology.||Transient Receptor Potential Vanilloid Subtype 1 Inhibits Inflammation and Apoptosis via the Release of Calcitonin Gene-Related Peptide in the Heart after Myocardial Infarction. Pubmed:27144592|
|J Headache Pain||Valproate ameliorates nitroglycerin-induced migraine in trigeminal nucleus caudalis in rats through inhibition of NF-кB Pubmed:27150105|
|Frontiers in Physiology||Prevention of Bone Loss in a Model of Postmenopausal Osteoporosis throughAdrenomedullin Inhibition. pubmed:27445864|
|Naunyn Schmiedebergs Arch Pharmacol.||Opioid receptor agonists may favorably affect bone mechanical properties in rats with estrogen deficiency-induced osteoporosis. pubmed:27896372|
|American Journal of Translational Research||A new comorbidity model and the common pathological mechanisms of migraine and epilepsy pubmed:28559979|
|9||Probiotic Bifidobacterium longum NCC3001 Reduces Depression Scores and Alters Brain Activity: A Pilot Study in Patients With Irritable Bowel Syndrome pubmed:28483500|
|Journal of Clinical Investigation||CNS myeloid cells critically regulate heat hyperalgesia Pubmed:29634489|
|Journal of Headache and Pain||Feasibility of serum CGRP measurement as a biomarker of chronic migraine: a critical reappraisal Pubmed:30006780|
|BioRxiv - the preprint server for biology||RAMP1 in Kupffer cells is a critical regulator in immune-mediated hepatitis 10.1101:357582|
|International Journal of Pharmaceutical Sciences and Research||MOTION SICKNESS INDUCES PHYSIOLOGICAL AND NEURONAL ALTERATIONS IN MOUSE MODEL|
|JOURNAL OF PERIODONTOLOGY||Periodontal inflammation is related to increased serum calcitonin gene‐related peptide (CGRP) levels in patients with chronic migraine Pubmed: 31070784|
|Molecular and Cellular Biochemistry||Rhynchophylline attenuates migraine in trigeminal nucleus caudalis in nitroglycerin-induced rat model by inhibiting MAPK/NF-кB signaling Pubmed: 31420791|
|Journal of Headache and Pain||Electrical stimulation of the superior sagittal sinus suppresses A-type K+ currents and increases P/Q-and T-type Ca 2+ currents in rat trigeminal ganglion …|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
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