ELISA Kit for Endothelin 1 (EDN1)

ET1; PPET1; Preproendothelin-1; Big endothelin-1

Specificity

This assay has high sensitivity and excellent specificity for detection of Endothelin 1 (EDN1).
No significant cross-reactivity or interference between Endothelin 1 (EDN1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Endothelin 1 (EDN1) and the recovery rates were calculated by comparing the measured value to the expected amount of Endothelin 1 (EDN1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 98-105 102
EDTA plasma(n=5) 79-91 81
heparin plasma(n=5) 79-92 83

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endothelin 1 (EDN1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endothelin 1 (EDN1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Endothelin 1 (EDN1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-96% 88-96% 82-97% 78-91%
EDTA plasma(n=5) 97-105% 81-93% 92-101% 89-96%
heparin plasma(n=5) 97-105% 81-90% 99-105% 80-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Heart and Circulatory Physiology B-type natriuretic peptide, vascular endothelial growth factor, endothelin-1, and nitric oxide synthase in chronic mountain sickness APS: source
PLOS ONE CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation PlosOne: Source
36 Megakaryocytic leukemia 1 (MKL1) ties the epigenetic machinery to hypoxia-induced transactivation of endothelin-1 PubMed: PMC3695508
Clinics (Sao Paulo). Antifibrotic effects of crocetin in scleroderma fibroblasts and in bleomycin-induced sclerotic mice Pubmed: 24212843
Life Sci. Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat model Pubmed:25476829
Transpl Int. One or 4 h of “in‐house” reconditioning by machine perfusion after cold storage improve reperfusion parameters in porcine kidneys Pubmed:24977654
J Endocrinol Invest.? The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders Pubmed:25245337
Chemical Research in Toxicology Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation Pubmed:25580880
Journal of Surgical Research Pivotal role of mast cell carboxypeptidase A in mediating protection against small intestinal ischemia–reperfusion injury in rats after ischemic preconditioning Sciencedirect:S0022480414004909
Pharmaceutical Biology The promising effect of barberry (Zereshk) extract against experimental pulmonary microvascular remodeling and hypertension: A comparison with sildenafil PubMed: 26023989
A Periodical of the Hungarian Academy of Sciences The effect of high altitude on endothelial and vascular dysfunction markers in preeclamptic patients PubMed: 26690031
Chemical Research in Toxicology Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation PubMed: 25580880
Human & Experimental Toxicology An in vivo and in vitro study High-dosage Danshen injection induces peripheral vascular endothelial cells injury PubMed: 26078283
Artery Research Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndrome science:article
Transplant  proc Cardiovascular Risk Factors and Markers of Atherosclerosis in Stable Kidney TransplantRecipients. pubmed:27496444
Biomed Journal Association of endothelial dysfunction and cytotoxin-associated gene A-positive Helicobacter pylori in patients with cardiac syndrome X. pubmed:27884380
International Journal of Advanced Research in Biological Sciences Comparative studies on lens structure and function of newborn rats maternally treated with aspartame, monosodium glutamate and galactose pdfcopy:42552
Journal of Drug Metabolisn& Toxicology Abnormal Retinal Structure and Function of Mother Wistar Rats Supplemented Aspartame, Glutamate and Galactose 587e5b6508aed3826af45e2d.pdf
Polish Journal of Veterinary Sciences Antioxidant and anti-inflammatory activities of a commercial noni juice revealed by carrageenan-induced paw edema pubmed:27760029
Artery Research Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndrome article:S1872-9312(16)30085-0
Journal of Molecular Biomarkers J & Diagnosis Biochemical and Molecular Markers of Congenital and Senile Cataractous Lenses DOI: 10.4172/2155-9929.1000318
A Study of Effect of Experimental Pre-Eclampsia on Plasma Lipocalin-2 Level in Rats. AN=123819331
Journal of Functional Foods Antihypertensive effects of Ocimum gratissimumextract: Angiotensin-converting enzyme inhibitor in vitro and in vivo investigation 10.1016/j.jff.2017.05.033
Radiation Research Selective Insulin-like Growth Factor Resistance Associated with Heart Hemorrhages and Poor Prognosis in a Novel Preclinical Model of the Hematopoietic Acute … Pubmed:29809108
Scandinavian Journal of Clinical and Laboratory Investigation Isolated kidney perfusion: the influence of pulsatile flow Pubmed:29301417
Endokrynologia Polska Endothelin-1 (ET-1), N-terminal fragment of proatrial natriuretic peptide (NTpro-ANP) and tumor necrosis factor alpha (TNF-α) in children with primary hypertension … Pubmed: 30359461
Journal of Pharmacological Sciences Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome Pubmed: 30342783
Molecular Nutrition & Food Research Short‐Term Isocaloric Intake of a Fructose‐but not Glucose‐Rich Diet Affects Bacterial Endotoxin Concentrations and Markers of Metabolic Health in Normal Weight … Pubmed: 30570214
Journal of Cosmetic Dermatology Whitening effects of cosmetic formulation in the vascular component of skin pigmentation Pubmed: 31074159
Eurasian Journal of Clinical Sciences APASL Single Topic Conference DELTA HEPATITIS, 27-28 June 2019, Baku, Azerbaijan
Journal of Functional Foods Potent anti-inflammatory activity of polysaccharides extracted from Blidingia minima and their effect in a mouse model of inflammatory bowel disease
Catalog No. Related products for research use of Capra hircus; Caprine (Goat) Organism species Applications (RESEARCH USE ONLY!)
CEA482Cp ELISA Kit for Endothelin 1 (EDN1) Enzyme-linked immunosorbent assay for Antigen Detection.