ELISA Kit for Adrenomedullin (ADM)
AM; PAMP; Proadrenomedullin N-20 terminal peptide; ProAM N-terminal 20 peptide
- Product No.CEA220Po
- Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range12.35-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.21pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Adrenomedullin (ADM).
No significant cross-reactivity or interference between Adrenomedullin (ADM) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Adrenomedullin (ADM) and the recovery rates were calculated by comparing the measured value to the expected amount of Adrenomedullin (ADM) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenomedullin (ADM) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenomedullin (ADM) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Adrenomedullin (ADM) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Journal of Periodontal Research||Gingival crevicular fluid adrenomedullin level in individuals with and without diabetes mellitus type 2 Wiley: Source|
|J Periodontal Res.||Comparison of peri-implant crevicular fluid levels of adrenomedullin and human beta defensins 1 and 2 from mandibular implants with different implant stability quotient levels in nonsmoker patients Pubmed: 23898869|
|Journal of Biosciences||Short-term hypoxia/reoxygenation activates the angiogenic pathway in rat caudate putamen. Pubmed: 23660671|
|Journal of Translational Medicine||DNA methylation patterns in newborns exposed to tobacco in utero Pubmed:25623364|
|Pediatric Hematology and Oncology||Adrenomedullin—A New Marker in Febrile Neutropenia: Comparison With CRP and Procalcitonin PubMed: 26271020|
|Biogerontology.||Angiogenesis is VEGF-independent in the aged striatum of male rats exposed to acute hypoxia. pubmed:28501895|
|Journal of Clinical Investigation||Adrenomedullin and Galanin Responses to Orthostasis in Older Persons pubmed:28796366|
|Clinical Pathology and Medical Laboratory||ADRENOMEDULIN DI KARSINOMA PAYUDARA DENGAN METASTASIS :|
|Phytomedicine||Composition and cardioprotective effects of Primula veris L. solid herbal extract in experimental chronic heart failure Doi: 10.1016/j.phymed.2018.09.015|
|Catalog No.||Related products for research use of Sus scrofa; Porcine (Pig) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA220Po01||Recombinant Adrenomedullin (ADM)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA220Po01||Polyclonal Antibody to Adrenomedullin (ADM)||WB; IHC; ICC; IP.|
|MAA220Po21||Monoclonal Antibody to Adrenomedullin (ADM)||WB; IHC; ICC; IP.|
|CEA220Po||ELISA Kit for Adrenomedullin (ADM)||Enzyme-linked immunosorbent assay for Antigen Detection.|