ELISA Kit for Fibroblast Growth Factor 1, Acidic (FGF1)

ECGF; AFGF; ECGFA; ECGFB; FGF-Alpha; FGFA; HBGF1; ECGFB; HBGF1; Acidic Fibroblast Growth Factor; heparin-binding growth factor 1; Endothelial Cell Growth Factor, Beta

Specificity

This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 1, Acidic (FGF1).
No significant cross-reactivity or interference between Fibroblast Growth Factor 1, Acidic (FGF1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Fibroblast Growth Factor 1, Acidic (FGF1) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibroblast Growth Factor 1, Acidic (FGF1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-101 95
EDTA plasma(n=5) 94-101 97
heparin plasma(n=5) 91-98 95

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 1, Acidic (FGF1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 1, Acidic (FGF1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibroblast Growth Factor 1, Acidic (FGF1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 82-95% 80-88% 84-96%
EDTA plasma(n=5) 78-99% 89-96% 85-98% 92-102%
heparin plasma(n=5) 95-105% 96-104% 98-105% 80-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Tissue Engineering Part A Tissue Inhibitor of Metalloproteinase-1 and -3 Improves Cardiac Function in an Ischemic Cardiomyopathy Model Rat Pubmed:24814095
24 Fibroblast growth factor 1 ameliorates diabetic nephropathy by an anti-inflammatory mechanism. pubmed:28750927
Endocrine Connections Characterization of fibroblast growth factor 1 in obese children and adolescents Pubmed:29991637
Catalog No. Related products for research use of Bos taurus; Bovine (Cattle) Organism species Applications (RESEARCH USE ONLY!)
RPA032Bo01 Recombinant Fibroblast Growth Factor 1, Acidic (FGF1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA032Bo01 Polyclonal Antibody to Fibroblast Growth Factor 1, Acidic (FGF1) WB; IHC; ICC; IP.
MAA032Bo21 Monoclonal Antibody to Fibroblast Growth Factor 1, Acidic (FGF1) WB; IHC; ICC; IP.
SEA032Bo ELISA Kit for Fibroblast Growth Factor 1, Acidic (FGF1) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA032Bo ELISA Kit for Fibroblast Growth Factor 1, Acidic (FGF1) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA032Bo CLIA Kit for Fibroblast Growth Factor 1, Acidic (FGF1) Chemiluminescent immunoassay for Antigen Detection.