CLIA Kit for Albumin (ALB)

Serum albumin

Specificity

This assay has high sensitivity and excellent specificity for detection of Albumin (ALB).
No significant cross-reactivity or interference between Albumin (ALB) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Albumin (ALB) and the recovery rates were calculated by comparing the measured value to the expected amount of Albumin (ALB) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-98 92
EDTA plasma(n=5) 88-102 92
heparin plasma(n=5) 88-97 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Albumin (ALB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Albumin (ALB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Albumin (ALB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-97% 95-103% 79-98% 83-98%
EDTA plasma(n=5) 86-102% 82-91% 85-97% 88-102%
heparin plasma(n=5) 79-96% 88-95% 95-103% 78-92%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.

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Magazine Citations
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Journal of Developmental Origins of Health and Disease Maternal low-quality protein diet exerts sex-specific effects on plasma amino acid profile and alters hepatic expression of methyltransferases in adult rat offspring Pubmed:29582727
Medicine Electret filter collects more exhaled albumin than glass condenser: A method comparison based on human study Pubmed:29384875
Stem Cell Research & Therapy Therapeutic efficiency of human amniotic epithelial stem cell-derived functional hepatocyte-like cells in mice with acute hepatic failure Pubmed: 30463600
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biomedicine & pharmacotherapy Treatment with 3, 4-dihydroxyphenylethyl alcohol glycoside ameliorates sepsis-induced ALI in mice by reducing inflammation and regulating M1 polarization Pubmed: 31146107
biochemical and biophysical research communications Anti-proteinuria effect of antibody against ANGPTL3 coil-coiled domain on adriamycin-induced nephropathy in mice Pubmed: 31256934
Diabetes, Metabolic Syndrome and Obesity A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activation Pubmed: 31447572
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
NPB028Mu01 Native Albumin (ALB) Positive Control; Immunogen; SDS-PAGE; WB.
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PAB028Mu01 Polyclonal Antibody to Albumin (ALB) WB; IHC; ICC; IP.
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