CLIA Kit for Parathyroid Hormone (PTH)
iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin
- Product No.CCA866Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range7.81-2,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 2.65pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Parathyroid Hormone (PTH).
No significant cross-reactivity or interference between Parathyroid Hormone (PTH) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Parathyroid Hormone (PTH) and the recovery rates were calculated by comparing the measured value to the expected amount of Parathyroid Hormone (PTH) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Parathyroid Hormone (PTH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Parathyroid Hormone (PTH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Parathyroid Hormone (PTH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Substrate A||1×10mL||Substrate B||1×2mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.
|Biological trace element research||Elevation of PTH and PTHrp Induced by Excessive Fluoride in Rats on a Calcium-deficient Diet PubMed: 19915804|
|Basic nutritional investigation||Effects of duodenal redox status on calcium absorption and related genes expression in high-fat diet–fed mice ScienceDirect: S0899900709004808|
|The Korean Journal of Nutrition||Effects of a Low Calcium Diet and Oxalate Intake on Calcium Deposits in Soft Tissues and Bone Metabolism in Ovariectomized Rats KoreaMed: source|
|Acta Physiologica Hungarica||Whole body vibration is a safe exercise training method and induces no impaired alterations on rat plasma parameters Pubmed: 22173025|
|Toxicology and Industrial Health||The effect of supplementation of calcium, vitamin D, boron, and increased fluoride intake on bone mechanical properties and metabolic hormones in rat PubMed: 22782709|
|Indian Journal of Experimental Biology||Effect of consumption of fatty acids, calcium, vitamin D and boron with regular physical activity on bone mechanical properties and corresponding metabolic hormones in rats. PubMed: 22439438|
|Plos one||Is Gastrectomy-Induced High Turnover of Bone with Hyperosteoidosis and Increase of Mineralization a Typical Osteomalacia? PubMed: PMC3679169|
|Nutr Hosp.||Effect of the “protein diet” and bone tissue. Pubmed: 24483972|
|Clinical Cancer Research||Zoledronic acid has differential anti-tumour activity in the pre-and post-menopausal bone microenvironment in vivo Aacrjournals: Source|
|Nutricion Hospitalaria||Effect of the “protein diet” and bone tissue Aulamedica: Source|
|Academic Journal||Effect of whole body vibration on healthy rat plasma parameters Ebscohost: Source|
|J Proteome Res.||Biomarkers identified by urinary metabonomics for noninvasive diagnosis of nutritional rickets Pubmed:25051233|
|Medicine (Baltimore)||An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer PubMed: 26266370|
|J Nutr Sci Vitaminol (Tokyo)||The Impact of Different Amounts of Calcium Intake on Bone Mass and Arterial Calcification in Ovariectomized Rats PubMed: 26639847|
|The Korean Society of Pharmacognosy||Effects of Oryza sativa L. Aleurone Layer Extract on Bone Mineral Density and Bone-related Markers in the Ovariectomized Rat Article: Hksobf_2015_V46N2_167|
|J Bone Miner Res||Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats Pubmed:26175082|
|7||Cell-matrix signals specify bone endothelial cells during developmental osteogenesis. pubmed:28218908|
|PLOS ONE||Prostaglandin-E2 Mediated Increase in Calcium and Phosphate Excretion in a Mouse Model ofDistal Nephron Salt Wasting. pubmed:27442254|
|International Journal of Advanced Research in Biological Sciences||Effects of fatty acids, nutrients and whole body vibration on bone histomorphometry, mechanical properties and metabolic parameters in male rat 10.22192/ijarbs.2017.04.04.018|
|American Journal of Physiology-Renal Physiology||Chronic nicotine exposure reduces klotho expression and triggers different renal and hemodynamic responses in -haploinsufficient mice Pubmed:29363324|
|Tropical Animal Health and Production||Dietary supplementation for Santa Inês hair ewes on pasture at pre-and postpartum periods: dry matter intake, digestibility, milk production, and mineral metabolism Pubmed:29931604|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
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