Active Gremlin 1 (GREM1)
PIG2; CKTSF1B1; DAND2; IHG-2; DAND2; DRM; Increased in high glucose protein 2; Cell proliferation-inducing gene 2 protein; Cysteine knot superfamily 1, BMP antagonist 1
- Product No.APC128Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Buffer Formulation100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point9.6
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
For more details, please contact local distributors! US$ 720
For more details, please contact local distributors! US$ 1440
For more details, please contact local distributors! US$ 4320
For more details, please contact local distributors! US$ 10800
For more details, please contact local distributors!
Figure 1. Cell proliferation of MCF-7 cells after stimulated with GREM1.
Gremlin(GREM1) is an inhibitor in the TGF beta signaling pathway. GREM1and other BMP antagonists are important in the survival of cancer stroma survival and proliferation in some cancers. The protein expression is found in many cancers and is thought to play important roles in uterine cervix, lung, ovary, kidney, breast, colon, pancreas, and sarcoma carcinomas. A proliferation assay was conducted to detect the bioactivity of recombinant rat GREM1 using A549 cells. Briefly, A549 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM prior to the addition of various concentrations of GREM1. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly，10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 °C. Proliferation of A549 cells after incubation with GREM1 for 96h observed by inverted microscope was shown in Figure1. Cell viability was assessed by CCK-8(Cell Counting Kit-8 ) assay after incubation with recombinant GREM1 for 96h. The result was shown in Figure2. It was obvious that GREM1 significantly increased cell viability of A549 cells.(A)MCF-7 cells cultured in DMEM, stimulated with 0.001ng/ml GREM1 for 96h; (B)Unstimulated MCF7 cells cultured in DMEM for 96h.
Figure. Cell proliferation of MCF-7 cells after stimulated with GREM1.
Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- BCA Protein Quantification Kit
- Molecular Mass Marker for Protein
- Monoclonal Antibody Customized Service
- Polyclonal Antibody Customized Service
- Protein Activity Test Experiment Service
- Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
- Lentivirus Packaging Experiment Service
- Adenovirus Packaging Experiment Service
- Real Time PCR Experimental Service
|Lung||Intrinsic BMP Antagonist Gremlin-1 as a Novel Circulating Marker in Pulmonary Arterial Hypertension PubMed: 25926293|
|Tumour Biol||Overexpression of Gremlin promotes non-small cell lung cancer progression PubMed: 26392110|
|Int J Cardiol||Triple head-to-head comparison of fibrotic biomarkers galectin-3, osteopontin and gremlin-1 for long-term prognosis in suspected and proven acute heart failure patients PubMed: 26539964|
|International Journal of Cardiology||Triple head-to-head comparison of fibrotic biomarkers galectin-3, osteopontin and gremlin-1 for long-termprognosis in suspected and proven acute heart failure patients Pubmed:26539964|
|American Journal of Respiratory Cell and Molecular Biology||BMPR2 Mutation-independent Mechanisms of Disrupted BMP Signaling in IPAH Pubmed:27187737|
|J Rheumatol||GREM1 Is a Key ReCavia (Guinea pig )lator of Synoviocyte Hyperplasia and Invasiveness Pubmed:26834210|
|European cells & materials||The BMP2 variant L51P restores the osteogenic differentiation of human mesenchymal stromal cells in the presence of intervertebral disc cells. pubmed:28266688|
|Sci Rep.||Genomic reprograming analysis of the Mesothelial to Mesenchymal Transition identifies biomarkers in peritoneal dialysis patients. pubmed:28327551|
|American Journal of Respiratory Cell and Molecular Biology||Bone Morphogenic Protein Type 2 Receptor Mutation-Independent Mechanisms of Disrupted Bone Morphogenetic Protein Signaling in Idiopathic Pulmonary Arterial … rcmb.2015-0402OC|
|Molecular Medicine Reports||Gremlin-1 suppression increases BMP-2-induced osteogenesis of human mesenchymal stem cells pubmed:28260028|
|Cellular Haemostasis and Platelets||Platelets as a novel source of Gremlin-1: Implications for thrombo inflammation pubmed:27929199|
|Scientific Reports||Gremlin, A Potential Urinary Biomarker of Anca-Associated Crescentic Glomerulonephritis Pubmed: 31053735|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|APC128Ra01||Active Gremlin 1 (GREM1)||Cell culture; Activity Assays.|
|RPC128Ra01||Recombinant Gremlin 1 (GREM1)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAC128Ra01||Polyclonal Antibody to Gremlin 1 (GREM1)||WB; IHC; ICC; IP.|
|SEC128Ra||ELISA Kit for Gremlin 1 (GREM1)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|PGC128Ra01||Primer Pair for Gremlin 1 (GREM1)||PCR|