Active Activin A (ACVA)

ACV-A; Activin Beta A Beta A Homodimer

ACTIVITY TEST

Figure. The binding activity of ACVA with ACVR1.
Activin A (ACVA) is a dimer composed of two identical called activin βA. Activin βA and βB are identical to the two beta subunits of inhibin. Activin enhances FSH biosynthesis and secretion, and participates in the regulation of the menstrual cycle. Many other functions have been found to be exerted by activin, including roles in cell proliferation, differentiation, apoptosis, metabolism, homeostasis, immune response, wound repair, and endocrine function. Besides, Activin A Receptor Type I (ACVR1) has been identified as an interactor of ACVA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ACVA and recombinant human ACVR1. Briefly, ACVA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to ACVR1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ACVA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of ACVA and ACVR1 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA001Hu01 Recombinant Activin A (ACVA) Positive Control; Immunogen; SDS-PAGE; WB.
APA001Hu01 Active Activin A (ACVA) Cell culture; Activity Assays.
PAA001Hu01 Polyclonal Antibody to Activin A (ACVA) WB; ICC; IHC-P; IHC-F; ELISA.
SEA001Hu ELISA Kit for Activin A (ACVA) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA001Hu ELISA Kit for Activin A (ACVA) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA001Hu CLIA Kit for Activin A (ACVA) Chemiluminescent immunoassay for Antigen Detection.