Active Activin A (ACVA)

ACV-A; Activin Beta A Beta A Homodimer


Figure. The binding activity of ACVA with ACVR1.
Activin A (ACVA) is a dimer composed of two identical called activin βA. Activin βA and βB are identical to the two beta subunits of inhibin. Activin enhances FSH biosynthesis and secretion, and participates in the regulation of the menstrual cycle. Many other functions have been found to be exerted by activin, including roles in cell proliferation, differentiation, apoptosis, metabolism, homeostasis, immune response, wound repair, and endocrine function. Besides, Activin A Receptor Type I (ACVR1) has been identified as an interactor of ACVA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ACVA and recombinant human ACVR1. Briefly, ACVA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to ACVR1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ACVA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of ACVA and ACVR1 was shown in Figure 1, and this effect was in a dose dependent manner.


Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.


Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.


The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.



Magazine Citations
13 Secretory Products From Epicardial Adipose Tissue of Patients With Type 2 Diabetes Mellitus Induce Cardiomyocyte Dysfunction Ahajournals: Source
United States Patent Application Methods for Diagnosis and Treatment of Concussion or Brain Injury 2016:0103140
BMC Cancer. Activins and their related proteins in colon carcinogenesis: insights from early and advanced azoxymethane rat models of colon cancer. pubmed:27835986
Basic Clin Pharmacol Toxicol. Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling. pubmed:28032440
Basic & Clinical Pharmacology & Toxicology Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4 + T Helper 17(Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling doi:10.1111
Journal of Immunology MicroRNA-27b Modulates Inflammatory Response and Apoptosis during Mycobacterium tuberculosis Infection Pubmed:29661829
Journal of Vascular Surgery Expression of platelet-derived growth factor B is upregulated in patients with thoracic aortic dissection Pubmed:29685513
Acta Biochimica et Biophysica Sinica Toxic effects of arsenic trioxide on protoscoleces through ROS production, and Ca-ER stress-dependent apoptosis 10.1093:abbs
Poultry Science The effect of raw and fermented rapeseed cake on the metabolic parameters, immune status, and intestinal morphology of turkeys Pubmed:29917099
Food Bioscience Characterization of Phospholipids from Pacific Saury () Viscera and Their Neuroprotective Activity 10.1016:j.fbio.2018.06.002
Current Analytical Chemistry Single Wall Carbon Nanotube and Magnetic Bead Based Electrochemical Immunosensor for Sensitive Detection of Salivary Secretory Immunoglobulin A 10.2174:2211556006666170525161225
Journal of Veterinary Internal Medicine FRIDAY, NOVEMBER 3, 2017 11.30-12.0 BALLROOM AB :
Involvement of RBP4 in diabetic atherosclerosis and the role of vitamin D intervention :
Clin Oral Investig.  Gingival crevicular fluid levels of human beta-defensin-1 in type 2 diabetes mellitus and periodontitis Pubmed:29713888
Myocardial fibrosis in hypertrophic cardiomyopathy :
Protein biomarker panels for detecting colorectal cancer and advanced adenoma :
Endothelotropic activity of 4-hydroxy-3, 5-di-tret-butylcinnamic acid in the conditions of experimental cerebral ischemia :
Silencing of the CSNK2β gene by siRNA inhibits invasiveness and growth of MDA-MB-231 cells 10.1101:311092
Duchenne Muscular Dystrophy Flow Cytometry-Defined CD49d Expression in Circulating T-Lymphocytes Is a Biomarker for Disease Progression in Duchenne Muscular Dystrophy Pubmed:29067667
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA001Hu01 Recombinant Activin A (ACVA) Positive Control; Immunogen; SDS-PAGE; WB.
APA001Hu01 Active Activin A (ACVA) Cell culture; Activity Assays.
PAA001Hu01 Polyclonal Antibody to Activin A (ACVA) WB; ICC; IHC-P; IHC-F; ELISA.
SEA001Hu ELISA Kit for Activin A (ACVA) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA001Hu ELISA Kit for Activin A (ACVA) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA001Hu CLIA Kit for Activin A (ACVA) Chemiluminescent immunoassay for Antigen Detection.