CLIA Kit for Activin A (ACVA)

ACV-A; Activin Beta A Beta A Homodimer

Specificity

This assay has high sensitivity and excellent specificity for detection of Activin A (ACVA).
No significant cross-reactivity or interference between Activin A (ACVA) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Activin A (ACVA) and the recovery rates were calculated by comparing the measured value to the expected amount of Activin A (ACVA) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-96 92
EDTA plasma(n=5) 97-105 101
heparin plasma(n=5) 97-105 102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activin A (ACVA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activin A (ACVA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Activin A (ACVA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 92-103% 87-101% 96-105%
EDTA plasma(n=5) 80-93% 88-95% 78-92% 99-105%
heparin plasma(n=5) 89-102% 82-104% 92-99% 83-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

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Magazine Citations
13 Secretory Products From Epicardial Adipose Tissue of Patients With Type 2 Diabetes Mellitus Induce Cardiomyocyte Dysfunction Ahajournals: Source
J. Pharmacogn Article: Hksobf_2015_V46N2_167
United States Patent Application Methods for Diagnosis and Treatment of Concussion or Brain Injury 2016:0103140
BMC Cancer. Activins and their related proteins in colon carcinogenesis: insights from early and advanced azoxymethane rat models of colon cancer. pubmed:27835986
Basic Clin Pharmacol Toxicol. Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling. pubmed:28032440
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Journal of Vascular Surgery Expression of platelet-derived growth factor B is upregulated in patients with thoracic aortic dissection Pubmed:29685513
Acta Biochimica et Biophysica Sinica Toxic effects of arsenic trioxide on protoscoleces through ROS production, and Ca-ER stress-dependent apoptosis 10.1093:abbs
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Food Bioscience Characterization of Phospholipids from Pacific Saury () Viscera and Their Neuroprotective Activity 10.1016:j.fbio.2018.06.002
Current Analytical Chemistry Single Wall Carbon Nanotube and Magnetic Bead Based Electrochemical Immunosensor for Sensitive Detection of Salivary Secretory Immunoglobulin A 10.2174:2211556006666170525161225
Journal of Veterinary Internal Medicine FRIDAY, NOVEMBER 3, 2017 11.30-12.0 BALLROOM AB :
Universal Journal of Pharmaceutical Research SEROPREVALENCE OF ANTI-MANNOSE BINDING LECTIN AUTOANTIBODIES IN PATIENTS WITH RHEUMATOID ARTHRITIS IN SANA'A CITY-YEMEN 10.22270:ujpr.v3i2.138
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Journal of Geriatric Oncology Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumors Pubmed: 30952517
clinica chimica acta The performances of serum activins and follistatin in the diagnosis of ectopic pregnancy: A prospective case-control study Pubmed: 31647905
hindawi The Association between the Activin A Serum Level and Carotid Intima-Media Thickness in Chronic Kidney Disease Patients
Pathology & Oncology Research Profiling Activins and Follistatin in Colorectal Cancer According to Clinical Stage, Tumour Sidedness and Smad4 Status 34867090
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