Primary cell culture is the process of single cellisolation from embryos, tissues and peripheral blood through special separationmethod, in-vitro cell culture in suitable culture medium, cell survival, growthand reproduction in the sterile system at appropriate temperature under certainconditions. The ‘apgebra’ for primary cell culture refers to the times for cellculture. It is the process from in-vivo tissue extraction, cell inoculationculture to the first cell subculture. It usually takes 1-4 weeks. Below is theflow chart for the common process of primary cell culture.The cell is directly derived from living tissues.In-vitro culture is also as far as possible to simulate the body......2016-04-11

NGAL (neutrophilgelatinase-associated lipocalin) widely exists in human tissues, such asbronchial, stomach, small intestine, pancreas, kidney, prostate gland, thymus,etc.Inrecent years, it's found that NGAL is closely related to nephropathy. Manyexperimental and clinical data have shown that the expression of NGAL greatlyincreased after kidney injury. NGAL has been considered as a novel biochemicalmarker in early nephropathy. It issignificant toprepare thespecific anti-NGALantibody and establish its immune detection system.Cloud-CloneCorp. has developed recombinant human NGAL (RPB388Hu01), which is expressed in prokaryotic system. There are anti-NGALmonoclonal antibody (MAB388Hu22)......2016-04-11

Generally, protein could be used as immunogen based ontheir amino acid sequence. Moreover, protein will possess specific biologicalfunctions while they get spatial conformation, and various kinds of functionsof different proteins will lead to physical functions of organism. So, theresearch of active protein is a hot spot of biological and medical researchfield. As researchers need to use biological active proteins, but natural onesare difficult to obtain, so, most of the native proteins used are generatedform genetic engineering..There are several steps and expression methods to getproteins by genetic engineering. But, it is necessary to select gene sequence,expression system and activ......2016-04-11

Protein quantification isan indispensable part of the biology experiment, this technique has been widelyused in many fields and industries, including biology, food inspection,clinical examination, diagnosis. It is very common and important to make accurateand reliable quantitative analysis of protein in samples in the actualoperation process. Although so many protein quantitative methods can be usedfor the purpose, there is no a generaland ideal protein quantitive detection method, which is suitable for all kindssituations. It is because of the great variety protein type, diversity instructure, big variance in molecular weight and different functions. Now weintroduce the common protein......2016-04-11

Histone is a kind of strongly basic proteinexists in chromatin of eukaryotic cell nucleus[A1], which is the basic structure protein ofeukaryotic chromosome. The DNA is compressed by Histones and together withHistones to form nucleosomes. If there is no histone[A2], the chromosomal DNA will be unlocked and will be scattered longDNA chains.[A3]It has greatsignificances to have researches on Histones. The abnormal level of Histoneswill lead to diseases, such as chronic kidney disease, etc. Also Histone modificationis related to immune regulation, autoimmune diseases, cancer, neurologicaldiseases and endocrine diseases.There are six types ofHistones: H1, H2A, H2B, H3, H4 and archaeal Histo......2016-04-11

High Mobility GroupProtein 1, also known as amphotericin. It’s encoded by HMGB1 genes. It’s namedwith HMGB1 due to the high mobility in SDS-PAGE.Now we treat humanHMGB1 as an example to have a brief introduction on High Mobility Group Protein1.Protein:Like Histones, HMGB1is the most important chromatin protein, it plays significant roles inmaintaining and stabilizing nucleosome DNA recombination, replication,transcription, repair etc. In addition, it also functions in infection,inflammation and immune reactions. HMGB1 is also one of the focus ofinflammation injury. Some scholars proposed HMGB1 as a DNA vaccine adjuvant andcancer therapeutic target.According UniprotMaster HMGB1 link......2016-04-11