GlucosidaseAlpha, Acid (GaA) is a kind of amylum hydrolase enzyme, which catalyzes thehydrolysis of polysaccharides to produce alpha D- glucose. In addition, it alsopossesses transglycosylation activity, which can transfer the alpha -1,4-glycosidic bond to alpha-1,6- glycosidic bond or other form linkages, to obtainnonfermented isomalto- oligosaccharide, glycolipids and glycopeptide etc.. The gene,which encode the GAA, is located at chromosome 17. Defects in this gene maylead to deficiency of GaA and glycogen accumulation in skeletal muscle, smoothmuscle & cardiac muscle tissue as the glycogen can’t be hydrolyzed by GaA. Then it may cause severeneuromuscular disease. This lysosomal s......2016-05-12

Complements are mainly distributed inbody fluids and cell surface of human or animal. After activation, they couldmediate immune and inflammatory responses, they are also known as thecomplement system.Complement system consists of nearly 40kinds of ingredients, most of them are glycoproteins.There are 13 inherentcomponents, over 10 regulatory proteins, and more than 10 kinds of complementreceptors.Complementcan’t be saved in hot temperature,when it reaches 56 degrees, most of the complementswill lose the activity after 30 minutes. Complement is difficult to preserve,in freeze and dry environment, they can bestored in relatively long time.ComplementComponent 3(C3) is a core component.......2016-05-12

Mitogen-activated protein kinases(Mark) is a group of serine - threonine protein kinase that can be activated byvarious extracellular stimulation, such as cytokines, neurotransmitters,hormone, cell stress and cell adhesion,etc. By stepwise phosphorylation,signalpathway of Mark as a important signal system could amplify and transmit thesignal, regulating the activity of transcription factor and expression of thecorresponding genes, and finally cause cellular response. Cascade activation ofMark is the center of a variety of signal pathways, involved in a variety oflife processes, such as, cell division, differentiation and apoptosis. And italso plays a key role in bone and joint inflamma......2016-05-12

Currently,theworld’s most commonly used kit to detect the protein concentration is BCA protein assay kit and Bradford protein assay kit.And BCA method alwayswas knows as simple,stable and sensitive. Thecompatibility of protein concentration were determined by BCA method is alsovery good.It is not affected by most of other components in the sample.For lessthan 5% of SDS,Triton X-100,Tween 20,Tween 80, it has good compatibility.Thereis a good linear relationship in the 50~1000μg/ml concentrationrange,and the minimum detection limit was 25μg/ml. Certainly,Cloud-Clone Corp. alsodeveloped its own BCA Protein Assay Kit.Now, The Cloud-Clone Crop. kits werecompared with Thermo kit by stand......2016-05-12

Recently,researches on the protein post-translational modification have becomea hot spotin area of the proteomics.The protein post-translational modification referringsome amino acids in the protein have been modified. There are kinds of proteinmodification,eg, Phosphorylation, glycosylation, methylation,acetylation, etc.Theprotein post-translational modification is one of the most important proteinmodifications.The protein post-translational modification consists of proteinkinases and phosphatases,which take part in a reversible process.It has foundthat the protein post-translational modification join in variety ofphysiological and pathological processes in vivo, such as glucose metab......2016-04-11

WithPCR technology develops, The door to the study of the essence of life hasopened.The PCR technology has become one of the most important technologies inthe last century.With the PCR ,we can amplify genes in vitro. Owning the targetgene fragment is the beginning for genetic modification,we need an efficienttool for gene processing----Gene-splicing techniques.In experiments,Gene-splicing techniques are play an impoertent role in them,eg: tranforminggene,tranforming plasmid, and tranforming species. In the 1989 ,Horton, etal. proposed the SEO technology.Geneswere spliced by over lap extension. In the 1991, Lebedenko et al. devised the SDLtechnology. Genes were spliced by directed ligat......2016-04-11