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Wide-range ELISA Kit for C-Peptide (CP)
- Product No.WEA447Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length3h
- Detection Range98.77-8,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 37.24pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 405
US$ 578
US$ 2601
US$ 4913
US$ 40460
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Wide-range C-Peptide (CP).
No significant cross-reactivity or interference between Wide-range C-Peptide (CP) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Wide-range C-Peptide (CP) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range C-Peptide (CP) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 93-101 | 96 |
| EDTA plasma(n=5) | 80-98 | 82 |
| heparin plasma(n=5) | 80-99 | 91 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range C-Peptide (CP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range C-Peptide (CP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range C-Peptide (CP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-105% | 89-101% | 86-94% | 96-103% |
| EDTA plasma(n=5) | 93-101% | 79-90% | 80-101% | 92-99% |
| heparin plasma(n=5) | 88-105% | 89-101% | 84-102% | 88-95% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1 | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| Reagent Diluent | 1×300µL | Stop Solution | 1×6mL |
| TMB Substrate | 1×9mL | Instruction manual | 1 |
| Wash Buffer (30 × concentrate) | 1×20mL |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
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| Magazine | Citations |
| Journal of Animal Science | The effect of oral and intravenous dextrose on C-peptide secretion in ponies Pubmed:27065127 |
| Medical Science Monitor | Increased β-Cell Mass in Obese Rats after Gastric Bypass: A Potential Mechanism for Improving Glycemic Control. pubmed:28477035 |
| Journal of Alzheimers Disease | Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental Methods Pubmed:30040720 |
| Diabetes-Metabolism Research and Reviews | Liraglutide Combined with Human Umbilical Cord Mesenchymal Stem Cell Transplantation inhibits beta‐cells apoptosis via mediating the ASK1/JNK/BAX pathway in Pubmed: 31411368 |
| Journal of dermatological treatment | Liraglutide improved inflammation via mediating IL-23/Th-17 pathway in obese diabetic mice with psoriasiform skin Pubmed: 31868553 |
| Journal of Diabetes Research | Roux-en-Y Gastric Bypass in Obese Diabetic Rats Promotes Autophagy to Improve Lipid Metabolism through mTOR/p70S6K Signaling Pathway Pubmed: 32309446 |
| Brain Research | Electroacupuncture improves cognitive deficits and insulin resistance in an OLETF rat model of Al/D-gal induced aging model via the PI3K/Akt signaling pathway Pubmed: 32304687 |
| Cancer Cell Int | Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer 33602229 |
| EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY | Different Ratios of Corn and Coconut Oil Blends in High©\Fat Diets Influence Fat Deposition without Altering Metabolic Biomarkers in Male Rats |
| Scientific Reports | SARS-CoV2 Induced Biochemical Mechanisms in Liver Damage and Intestinal Lesions. |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| CPA447Ra21 | OVA Conjugated C-Peptide (CP) | Immunogen; SDS-PAGE; WB. |
| CPA447Ra31 | KLH Conjugated C-Peptide (CP) | Immunogen; SDS-PAGE; WB. |
| CPA447Ra11 | BSA Conjugated C-Peptide (CP) | Immunogen; SDS-PAGE; WB. |
| PAA447Ra08 | Polyclonal Antibody to C-Peptide (CP) | WB; IHC; ICC; IP. |
| MAA447Ra21 | Monoclonal Antibody to C-Peptide (CP) | WB; IHC; ICC; IP. |
| MAA447Ra22 | Monoclonal Antibody to C-Peptide (CP) | WB; IHC; ICC; IP. |
| MAA447Ra28 | Monoclonal Antibody to C-Peptide (CP) | WB; IHC; ICC; IP. |
| MAA447Ra24 | Monoclonal Antibody to C-Peptide (CP) | WB; IHC; ICC; IP. |
| MAA447Ra23 | Monoclonal Antibody to C-Peptide (CP) | WB; IHC; ICC; IP. |
| CEA447Ra | ELISA Kit for C-Peptide (CP) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA447Ra | Mini Samples ELISA Kit for C-Peptide (CP) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| WEA447Ra | Wide-range ELISA Kit for C-Peptide (CP) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA447Ra | CLIA Kit for C-Peptide (CP) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA447Ra | Multiplex Assay Kit for C-Peptide (CP) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSA447Ra11 | ELISA Kit DIY Materials for C-Peptide (CP) | Main materials for "Do It (ELISA Kit) Yourself". |