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ELISA Kit for Autophagy Related Protein 5 (ATG5)
ASP; APG5; APG5L; hAPG5; Apoptosis-specific protein; Autophagy protein 5
- Product No.SEL221Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typetissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.059ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 585
For more details, please contact local distributors! US$ 836 US$ 3762 US$ 7106 US$ 58520
Specificity
This assay has high sensitivity and excellent specificity for detection of Autophagy Related Protein 5 (ATG5).
No significant cross-reactivity or interference between Autophagy Related Protein 5 (ATG5) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Autophagy Related Protein 5 (ATG5) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Autophagy Related Protein 5 (ATG5) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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Magazine | Citations |
Frontiers in Cellular Neuroscience | A Variant of the Autophagy-Related 5 Gene Is Associated with Child Cerebral Palsy. pubmed:29326554 |
Medical Science Monitor | Relationship of Autophagy and Apoptosis with Total Occlusion of Coronary Arteries Pubmed: 30273932 |
Scientific Reports | Plasma ATG5 is increased in Alzheimer's disease |
Acta Neurol Belg | Association of ATG5 gene polymorphism with Parkinson's disease in a Han Chinese population 34661876 |
Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
SEL221Ra | ELISA Kit for Autophagy Related Protein 5 (ATG5) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LML221Ra | Multiplex Assay Kit for Autophagy Related Protein 5 (ATG5) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |