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ELISA Kit for Retinoschisin (RS)
RS1; XLRS1; Retinoschisis X-Linked,Juvenile 1; X-linked juvenile retinoschisis protein
- Product No.SEJ847Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typetissue homogenates, cell lysates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.059ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 490
For more details, please contact local distributors! US$ 700 US$ 3150 US$ 5950 US$ 49000
Specificity
This assay has high sensitivity and excellent specificity for detection of Retinoschisin (RS).
No significant cross-reactivity or interference between Retinoschisin (RS) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Retinoschisin (RS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Retinoschisin (RS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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Magazine | Citations |
Hum Gene Ther | Dextran and protamine-based solid lipid nanoparticles as potential vectors for the treatment of X-linked juvenile retinoschisis. PubMed: 22295905 |
IEEE Transactions on Magnetics | Influence of Iron Oxide Nanoparticles on Innate and Genetically Modified Secretion Profiles of Mesenchymal Stem Cells Scholarsportal: Source |
International Journal of Pharmaceutics | A novel gene therapy vector based on hyaluronic acid and solid lipid nanoparticles for ocular diseases Pubmed:24576595 |
22 | Structural recovery of the retina in a retinoschisin-deficient mouse after gene replacement therapy by solid lipid nanoparticles Pubmed:26986855 |
UBC Library | Cell based therapeutics for retinal degenerations cIRcle:1.0303136 |
Molecular Vision | An ex vivo gene therapy approach in X-linked retinoschisis pubmed:27390514 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPJ847Hu01 | Recombinant Retinoschisin (RS) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAJ847Hu01 | Polyclonal Antibody to Retinoschisin (RS) | WB |
MAJ847Hu21 | Monoclonal Antibody to Retinoschisin (RS) | WB; IHC; ICC; IP. |
SEJ847Hu | ELISA Kit for Retinoschisin (RS) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCJ847Hu | CLIA Kit for Retinoschisin (RS) | Chemiluminescent immunoassay for Antigen Detection. |
LMJ847Hu | Multiplex Assay Kit for Retinoschisin (RS) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |