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ELISA Kit for Cystathionine Beta Synthase (CbS)
HIP4; Beta-thionase; Serine sulfhydrase
- Product No.SEJ484Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample Typetissue homogenates, cell lysates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.312-20ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.122ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
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For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Cystathionine Beta Synthase (CbS).
No significant cross-reactivity or interference between Cystathionine Beta Synthase (CbS) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cystathionine Beta Synthase (CbS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cystathionine Beta Synthase (CbS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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Magazine | Citations |
Journal of Functional Foods | Effects of maternal serine supplementation on high-fat diet-induced oxidative stress and epigenetic changes in promoters of glutathione synthesis-related genes in … 10.1016:j.jff.2018.05.067 |
Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
RPJ484Mu02 | Recombinant Cystathionine Beta Synthase (CbS) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPJ484Mu01 | Recombinant Cystathionine Beta Synthase (CbS) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAJ484Mu02 | Polyclonal Antibody to Cystathionine Beta Synthase (CbS) | WB; IHC; ICC; IP. |
PAJ484Mu01 | Polyclonal Antibody to Cystathionine Beta Synthase (CbS) | WB; IHC; ICC; IP. |
SEJ484Mu | ELISA Kit for Cystathionine Beta Synthase (CbS) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMJ484Mu | Multiplex Assay Kit for Cystathionine Beta Synthase (CbS) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |