≡

ELISA Kit for Receptor Interacting Serine Threonine Kinase 3 (RIPK3)

RIP3; RIP-like protein kinase 3; Receptor-interacting protein 3

Specificity

This assay has high sensitivity and excellent specificity for detection of Receptor Interacting Serine Threonine Kinase 3 (RIPK3).
No significant cross-reactivity or interference between Receptor Interacting Serine Threonine Kinase 3 (RIPK3) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor Interacting Serine Threonine Kinase 3 (RIPK3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor Interacting Serine Threonine Kinase 3 (RIPK3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Atherosclerosis Vascular peroxide 1 promotes ox-LDL-induced programmed necrosis in endothelial cells through a mechanism involving β-catenin signaling Pubmed:29772481
Science Signaling Inflammation, necrosis, and the kinase RIP3 are key mediators of AAG-dependent alkylation-induced retinal degeneration Pubmed: 30755477
TOXICOLOGY LETTERS Protective effect of ISO-1 with inhibition of RIPK3 up-regulation and neutrophilic accumulation on acetaminophen-induced liver injury in mice 33370591
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPE639Hu01 Recombinant Receptor Interacting Serine Threonine Kinase 3 (RIPK3) Positive Control; Immunogen; SDS-PAGE; WB.
PAE639Hu01 Polyclonal Antibody to Receptor Interacting Serine Threonine Kinase 3 (RIPK3) WB; IHC; ICC; IP.
MAE639Hu21 Monoclonal Antibody to Receptor Interacting Serine Threonine Kinase 3 (RIPK3) WB; IHC; ICC; IP.
SEE639Hu ELISA Kit for Receptor Interacting Serine Threonine Kinase 3 (RIPK3) Enzyme-linked immunosorbent assay for Antigen Detection.
LME639Hu Multiplex Assay Kit for Receptor Interacting Serine Threonine Kinase 3 (RIPK3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.