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ELISA Kit for Serotonin Transporter (SERT)
SLC6A4; SLC6-A4; HTT; 5-HTT; 5HTT; OCD1; hSERT; Solute Carrier Family 6,Member 4; Neurotransmitter Transporter,Serotonin; Sodium-dependent serotonin transporter
- Product No.SEC890Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typetissue homogenates, cell lysates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.057ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 532
For more details, please contact local distributors! US$ 760 US$ 3420 US$ 6460 US$ 53200
Specificity
This assay has high sensitivity and excellent specificity for detection of Serotonin Transporter (SERT).
No significant cross-reactivity or interference between Serotonin Transporter (SERT) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serotonin Transporter (SERT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serotonin Transporter (SERT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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Magazine | Citations |
Journal of Prosthodontic Research | Comparison of platelet serotonin transporter activity in subjects with severe sleep bruxism and control Pubmed:25127373 |
Frontiers in Behavioral Neuroscience? | Experimental heart failure causes depression-like behavior together with differential regulation of inflammatory and structural genes in the brain Pubmed:25400562 |
Sleep Breath | Sleep bruxism frequency and platelet serotonin transporter activities in young adult subjects PubMed: 26527205 |
PLoS One. | Non-Serotonergic Neurotoxicity by MDMA (Ecstasy) in Neurons Derived fromMouse P19 Embryonal Carcinoma Cells. pubmed:27861613 |
Herz-Hirn Interaktion im Mausmodell: Herzinsuffizienz nach Myokardinfarkt führt zu depressivem Verhalten bei Mäusen frontdoor:index | |
Effects of Junk Foods on Brain Neurotransmitters (Dopamine and Serotonin) and some Biochemical Parameters in Albino Rats article_1486_c0fa9f8afe5f70b407e7195bcfd55c70 | |
Toxicology Reports | Neuro-and nephroprotective effect of grape seed proanthocyanidin extract against carboplatin and thalidomide through modulation of inflammation, tumor … Pubmed:29854627 |
Journal of Taibah University for Science | Neurotoxicity and inflammation induced by individual and combined exposure to iron oxide nanoparticles and silver nanoparticles |
NEUROGASTROENTEROLOGY AND MOTILITY | Effects of regulating gut microbiota on the serotonin metabolism in the chronic unpredictable mild stress rat model Pubmed: 31323174 |
Thrombosis Research | VAMP8 and serotonin transporter levels are associated with venous thrombosis risk in a Spanish female population. Results from the RETROVE Project Pubmed: 31382081 |
Scientia Psychiatrica | Neuronal Cell Death Induces Depressive Disorder in Rats Depression-Like Behaviors Caused by Chronic Stress |
Journal of Immunology | Administration of a Probiotic Mixture Ameliorates Cisplatin-Induced Mucositis and Pica by Regulating 5-HT in Rats 34568500 |
Biol Pharm Bull | Targeting the oxytocin system to ameliorate early life depressive-like behaviors in maternally-separated rats 34349049 |
Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
RPC890Ra01 | Recombinant Serotonin Transporter (SERT) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAC890Ra01 | Polyclonal Antibody to Serotonin Transporter (SERT) | WB; IHC; ICC; IP. |
MAC890Ra21 | Monoclonal Antibody to Serotonin Transporter (SERT) | WB; IHC; ICC; IP. |
SEC890Ra | ELISA Kit for Serotonin Transporter (SERT) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMC890Ra | Multiplex Assay Kit for Serotonin Transporter (SERT) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
KSC890Ra01 | ELISA Kit DIY Materials for Serotonin Transporter (SERT) | Main materials for "Do It(ELISA Kit) Yourself" |